Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Apr 26, 2021; 13(4): 317-330
Published online Apr 26, 2021. doi: 10.4252/wjsc.v13.i4.317
Hypoxia-inducible factor-1α–mediated upregulation of CD99 promotes the proliferation of placental mesenchymal stem cells by regulating ERK1/2
Xu-Dong Feng, Jia-Qi Zhu, Jia-Hang Zhou, Fei-Yan Lin, Bing Feng, Xiao-Wei Shi, Qiao-Ling Pan, Jiong Yu, Lan-Juan Li, Hong-Cui Cao
Xu-Dong Feng, Jia-Qi Zhu, Jia-Hang Zhou, Fei-Yan Lin, Bing Feng, Xiao-Wei Shi, Qiao-Ling Pan, Jiong Yu, Lan-Juan Li, Hong-Cui Cao, State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, China
Author contributions: Feng XD contributed to the experimental design, cellular and molecular biology experiments, and manuscript writing; Zhu JQ, Zhou JH and Lin FY contributed to the cellular experiment; Feng B and Shi XW joined in the molecular biology experiment; Feng XD, Pan QL and Yu J participated in analysis, interpretation of data and manuscript writing; Li LJ and Cao HC participated in the conception, design, study supervision, and manuscript writing; all authors reviewed and approved the final version of the manuscript.
Supported by Stem Cell and Translational Research from the National Key Research and Development Program of China, No. 2020YFA0113003; and National Natural Science Foundation of China, No. 81971756.
Institutional review board statement: The study was reviewed and approved by the Research Ethics Committee of The First Affiliated Hospital, College of Medicine, Zhejiang University Institutional Review Board (Approval No. 2013-272).
Conflict-of-interest statement: The authors declare that they have no conflicts of interest.
Data sharing statement: The data used to support the findings of this study are available from the corresponding authors upon request.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hong-Cui Cao, MD, PhD, Professor, State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. hccao@zju.edu.cn
Received: February 1, 2021
Peer-review started: February 1, 2021
First decision: February 28, 2021
Revised: March 11, 2021
Accepted: March 29, 2021
Article in press: March 29, 2021
Published online: April 26, 2021
Processing time: 80 Days and 6.7 Hours
Core Tip

Core Tip: This study demonstrated the potential of hypoxic conditions to enhance the proliferation capacity of human placenta-derived mesenchymal stem cells (hP-MSCs). RNA sequencing assays and molecular biology experiments found that a novel CD99 gene was involved in hypoxia-mediated promotion of hP-MSC proliferation. Furthermore, CD99 activated pathways that transported ERK1/2 to the nucleus, increasing the activity of cell cycle-associated proteins. Hypoxia-inducible factor 1α-mediated CD99 played a role in proliferation by regulating the MAPK/ERK signaling pathway.