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Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Oct 26, 2021; 13(10): 1394-1416
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1394
Alternative RNA splicing in stem cells and cancer stem cells: Importance of transcript-based expression analysis
Esmaeil Ebrahimie, Samira Rahimirad, Mohammadreza Tahsili, Manijeh Mohammadi-Dehcheshmeh
Esmaeil Ebrahimie, Manijeh Mohammadi-Dehcheshmeh, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide 5005, South Australia, Australia
Esmaeil Ebrahimie, La Trobe Genomics Research Platform, School of Life Sciences, College of Science, Health and Engineering, La Trobe University, Melbourne 3086, Australia
Esmaeil Ebrahimie, School of Biosciences, The University of Melbourne, Melbourne 3010, Australia
Samira Rahimirad, Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran 1497716316, Iran
Samira Rahimirad, Division of Urology, Department of Surgery, McGill University and the Research Institute of the McGill University Health Centre, Montreal H4A 3J1, Quebec, Canada
Mohammadreza Tahsili, Department of Biology, University of Qom, Qom 3716146611, Iran
Author contributions: Ebrahimie E and Rahimirad S contributed equally in this study; Ebrahimie E conceived the idea of this manuscript; Rahimirad S, Tahsili M performed the literature review and initial data collection; Ebrahimie E, Rahimirad S wrote the manuscript; Mohammadi-Dehcheshmeh M performed figure preparation; Ebrahimie E, Mohammadi-Dehcheshmeh M edited and prepared the manuscript for submission; all authors have read and approved the final manuscript.
Conflict-of-interest statement: The authors declare no conflict of interest for this article.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Esmaeil Ebrahimie, PhD, Associate Professor, School of Animal and Veterinary Sciences, The University of Adelaide, No. 1 Mudla Wirra Rd, Roseworthy Campus, Adelaide 5005, South Australia, Australia. esmaeil.ebrahimie@adelaide.edu.au
Received: March 18, 2021
Peer-review started: March 18, 2021
First decision: June 16, 2021
Revised: June 21, 2021
Accepted: September 14, 2021
Article in press: September 14, 2021
Published online: October 26, 2021
Processing time: 221 Days and 18 Hours
Core Tip

Core Tip: The alternative splicing machinery can produce various variants, associated with stemness characteristics of both stem cells (SCs) and cancer SCs. In this study, the role of spliced variants in SCs and stem-like cancer cells is reviewed. We highlight the importance of transcript-based expression concurrent with the gene and protein expression that leads to better understanding of self-renewal features of tumor cells.