Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jan 26, 2018; 10(1): 1-14
Published online Jan 26, 2018. doi: 10.4252/wjsc.v10.i1.1
Rat model of anal sphincter injury and two approaches for stem cell administration
Jacobo Trébol, Tihomir Georgiev-Hristov, Luz Vega-Clemente, Ignacio García-Gómez, Ana Carabias-Orgaz, Mariano García-Arranz, Damián García-Olmo
Jacobo Trébol, Department of General and Digestive Tract Surgery, University Hospital “La Paz”, Madrid 28046, Spain
Tihomir Georgiev-Hristov, Department of General and Digestive Tract Surgery, Villalba General Hospital, Madrid 28400, Spain
Luz Vega-Clemente, New Therapies Laboratory, Instituto de Investigación Sanitaria- Fundación Jiménez Díaz, Madrid 28040, Spain
Ignacio García-Gómez, Senior Research Associate, Hektoen Institute of Medicine, Chicago, Illinois 60612, United States
Ana Carabias-Orgaz, Department of Anaesthesiology, Complejo Asistencial de Ávila, Ávila 05004, Spain
Mariano García-Arranz, Scientific Head, New Therapies Laboratory, Instituto de Investigación Sanitaria- Fundación Jiménez Díaz, Madrid 28040, Spain
Damián García-Olmo, Head of Department, Department of General and Digestive Tract Surgery, Quiron-Salud Hospitals, Madrid 28040, Spain
Damián García-Olmo, Department of Surgery, Madrid Autonomous University, Madrid 28029, Spain
Author contributions: Trébol J performed the majority of experiments, and analyzed and interpreted the data; Georgiev-Hristov T and Vega-Clemente L collaborated with experiments and animal management; García-Gómez I collaborated performing immunofluorescence investigations; Carabias-Orgaz A revised the anesthetic methodology; García-Arranz M and García-Olmo D designed and coordinated the research, and obtained financial support; Trébol J, Georgiev-Hristov T and Carabias-Orgaz A wrote the paper; all authors reviewed the paper and gave their final approval to the manuscript.
Supported by Spanish Ministry of Health and Consumer Affairs, No. PI060305.
Institutional review board statement: Experimental protocols were approved by the Ethical Welfare Animal Committee from La Paz University Hospital, Madrid, Spain.
Institutional animal care and use committee statement: Experimental protocols were approved by the Ethical Welfare Animal Committee from La Paz University Hospital, Madrid, Spain. All the experimental work was done in the Cell Therapy and Genometastasis Laboratory and Experimental Surgery Area of the La Paz Investigation Institute (IdiPAZ) under the supervision of the corresponding responsible personnel.
Conflict-of-interest statement: Damián García-Olmo is a member of the Advisory Board of Tigenix S.A.U. and co-holds patent rights to biomaterial for suturing (P200402083–Spain-, 04380271.9–Europe and 101573.55823US-United States-). All other authors indicated no potential conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Jacobo Trébol, MD, PhD, Department of General and Digestive Tract Surgery, University Hospital “La Paz”, Paseo de la Castellana, nº 261, Madrid 28046, Spain. jacobo.trebol@salud.madrid.org
Telephone: +34-91-7271667 Fax: +34-91-7277050
Received: August 13, 2017
Peer-review started: August 14, 2017
First decision: September 25, 2017
Revised: October 26, 2017
Accepted: November 27, 2017
Article in press: November 27, 2017
Published online: January 26, 2018
Processing time: 164 Days and 13.3 Hours
Core Tip

Core tip: Fecal incontinence is very frequent and associated with severe consequences for patients. Surgical treatment outcomes are not as good as they should be, mainly in the long term. Stem cells could improve these results, as demonstrated in other clinical settings. We report a simple rat model for experimental anal sphincter injury (a surgical section), and characterize it from morphological (conventional histology and immunofluorescence) and functional (anal manometry) points of view. Then, we describe two approaches for adipose-derived stem cell administration to the injured area (injection and biosutures) and demonstrate stem cell survival during at least 7 d, as well as their safety.