Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Nov 26, 2020; 12(11): 1396-1409
Published online Nov 26, 2020. doi: 10.4252/wjsc.v12.i11.1396
Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis
Gui-Long Zhang, Cheng Qian, Shi-Zhen Zhang, Yong-Hua Tuo, Bai-Yun Zeng, Yun-Xiang Ji, Ye-Zhong Wang
Gui-Long Zhang, Cheng Qian, Shi-Zhen Zhang, Yong-Hua Tuo, Bai-Yun Zeng, Yun-Xiang Ji, Ye-Zhong Wang, Department of Neurosurgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong Province, China
Gui-Long Zhang, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province China
Author contributions: Zhang GL, Qian C, and Zhang SZ designed and performed the experiments; Ji YX and Wang YZ contributed to data analysis; Zhang SZ, Tuo YH, and Zeng BY performed the experiments; Zhang GL, Qian C, and Zhang SZ wrote the paper, Ji YX and Wang YZ edited the manuscript; all authors have approved the final manuscript; Zhang GL, Qian C, and Zhang SZ contributed equally to this work.
Supported by China Postdoctoral Science Foundation, No. 2020M672592 and No. 2019TQ0071.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at Affiliated Zhongda Hospital of Southeast University.
Conflict-of-interest statement: The authors have no conflicts of interest to disclose.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Ye-Zhong Wang, MD, PhD, Chairman, Chief Doctor, Professor, Department of Neurosurgery, The Second Affiliated Hospital of Guangzhou Medical University, No. 250 East Changgang Road, Haizhu District, Guangzhou 510260, Guangdong Province, China. wangyezhong@gzhmu.edu.cn
Received: June 1, 2020
Peer-review started: June 1, 2020
First decision: August 22, 2020
Revised: September 4, 2020
Accepted: September 25, 2020
Article in press: September 25, 202
Published online: November 26, 2020
Processing time: 178 Days and 3.5 Hours
ARTICLE HIGHLIGHTS
Research background

Glioma contacting with the subventricular zone (SVZ) is associated with a poor prognosis and decreased overall survival of patients. The most important cell type in the SVZ region is neural stem cells (NSCs), which can differentiate into neurons, astrocytes, and oligodendrocytes.

Research motivation

The roles of neural stem cells of the subventricular zone (SVZ NSCs) in glioma progression are still unclear.

Research objectives

To investigate the effects of SVZ NSCs in gliomas, especially the conditioned medium (CM) derived from SVZ NSCs, on the tumor-related behaviors of glioma cells.

Research methods

We used the CM derived from SVZ NSCs under normoxic and hypoxic conditions to co-culture with glioma cells, and explored the potential functional molecules in the CM by mass spectrometry analysis.

Research results

After co-cultured with glioma cells, the CM derived from hypoxic SVZ NSCs further increased glioma cell proliferation and invasion compared with normoxic CM. The MS analysis identified the candidate protein NCAN in the hypoxic CM. Furthermore, NCAN played an important role in mediating the progression of tumor cells mainly via the Rho/ROCK pathway in gliomas.

Research conclusions

SVZ NSC-derived CM under hypoxic condition further increases glioma cell proliferation and invasion, and the protein NCAN secreted from hypoxic SVZ NSCs plays prominent regulatory effects in the progression of glioma cells.

Research perspectives

The study provided important insights into the mechanisms of tumor progression associated with glioma contacting SVZ region, and suggested a new molecular target of gene therapy for gliomas.