Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jan 26, 2020; 12(1): 55-69
Published online Jan 26, 2020. doi: 10.4252/wjsc.v12.i1.55
Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats' critical-sized mandibular defects
Muhammad A Awadeen, Fouad A Al-Belasy, Laila E Ameen, Mohamad E Helal, Mohammed E Grawish
Muhammad A Awadeen, Mohammed E Grawish, Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Mansoura 11152, Egypt
Fouad A Al-Belasy, Department of Oral Surgery and Anesthesia, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Mansoura 11152, Egypt
Laila E Ameen, Mohamad E Helal, Mohammed E Grawish, Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura 35516, Egypt
Author contributions: Awadeen MA generated the figures, tables and wrote the manuscript; Al-Belasy FA, Grawish ME, Ameen LE and Helal ME provided an intellectual contribution to the content, contributed to writing of the manuscript, and made all critical revisions; all authors read, reviewed and approved the final version of the manuscript.
Institutional animal care and use committee statement: All experimental procedures were performed under a protocol approved by the Ethics Committee of the Faculty of Dentistry, Mansoura University, Egypt. The rats were cared for and housed in standard cages by the guidelines of the Faculty of Medicine, Medical Research Centre, Mansoura University, Egypt.
Conflict-of-interest statement: Authors declare no conflict of interests for this article.
Data sharing statement: The datasets supporting the conclusions of this article are included in the article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Mohammed E Grawish, BDS, MSc, PhD, Professor, Department of Oral Biology, Faculty of Dentistry, Mansoura University, Dakahlia Governorate, Mansoura 35516, Egypt. grawish2005@yahoo.com
Received: March 7, 2019
Peer-review started: March 8, 2019
First decision: April 16, 2019
Revised: May 13, 2019
Accepted: November 29, 2019
Article in press: November 29, 2019
Published online: January 26, 2020
Processing time: 298 Days and 14 Hours
ARTICLE HIGHLIGHTS
Research background

Regeneration of critical-sized bone defects remains a major clinical problem in the field of orthopaedic surgery, and therefore, novel treatment methods must be developed. Currently, the management of such defects mainly depends on the use of autologous bone grafts. However, complications such as donor site morbidity drive us to find other lines of treatments.

Research motivation

To investigate whether allogenic bone marrow-derived stem cells (BMSCs) seeded on platelet-rich fibrin (PRF) membranes have the ability to regenerate critical-sized mandibular defects in rats and, therefore, whether this combination therapy is a suitable approach for developing a new line of treatment for such bony defects.

Research objectives

The objectives of the present study were to create critical-sized mandibular defects, to seed BMSCs on PRF membranes, to fill these defects with the combination therapy, and finally, to assess the possible regenerative effect of PRF membranes with or without allogeneic BMSCs on such bony defects in rat models.

Research methods

We induced critically sized defects and treated these defects with a combined therapy, followed by performing histological and immunohistochemical analyses. The data of the histomorphometric analysis were statistically analysed.

Research results

The percentage area of newly formed bone was significantly higher in the defects treated with the combined therapy than in the defects treated with the PRF membrane alone and untreated defects. However, the amount of granulation tissue formation and the number of inflammatory cells were lower in the defects treated with the combined therapy than in the defects treated with the PRF membrane alone.

Research conclusions

The combined therapy of BMSCs and PRF membrane showed a regenerative effect in critically sized bone defects and may represent a potential therapeutic alternative for bone regeneration.

Research perspectives

Based on our results, we believe that BMSCs seeded on platelet-rich plasma could be clinically applied for treating critically sized bone defects and promoting wound regeneration in the future.