©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
Characterization of inflammatory factor-induced changes in mesenchymal stem cell exosomes and sequencing analysis of exosomal microRNAs
Chen Huang, Wen-Feng Luo, Yu-Feng Ye, Qi-De Song, Xue-Ping He, Han-Wei Chen, Yu-Kuan Tang, Department of Minimally Invasive Interventional Radiology, Guangzhou Panyu Central Hospital, Guangzhou 511400, Guangdong Province, China
Chen Huang, Han-Wei Chen, Jinan University, Guangzhou 510632, Guangdong Province, China
Chen Huang, Wen-Feng Luo, Yu-Feng Ye, Han-Wei Chen, Medical Imaging Institute of Panyu, Guangzhou 511400, Guangdong Province, China
Li Lin, Jinan University Biomedical Translational Research Institute, Jinan University, Guangzhou 510632, Guangdong Province, China
Zhe Wang, Yi Kong, Department of Pharmacy, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong Province, China
Ming-Hua Luo, Department of Radiology, Shiyan People’s Hospital, Shenzhen 518108, Guangdong Province, China
Author contributions: Huang C designed the research and wrote the manuscript; Huang C, Luo WF, and Lin L performed all the experiments; Huang C, Wang Z, Kong Y, Chen HW, and Tang YK performed the analysis; Ye YF, He XP, Luo MH, and Song QD performed data collection and pre-processing; all authors approved the manuscript.
Supported by Panyu Science and Technology Plan Medical General Project, No. 2018-Z04-47; and Guangzhou Health Science and Technology Project, No. 20191A011120.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Yu-Kuan Tang, MD, Doctor, Department of Minimally Invasive Interventional Radiology, Guangzhou Panyu Central Hospital, 8 East Fuyu Road, Qiaonan Street, Panyu District, Guangzhou 511400, Guangdong Province, China. firstname.lastname@example.org
Telephone: +86-20-34858815 Fax: +86-20-84824443
Received: January 29, 2019
Peer-review started: January 29, 2019
First decision: March 14, 2019
Revised: March 24, 2019
Accepted: July 30, 2019
Article in press: July 30, 2019
Published online: October 26, 2019
Stem cell transplantation has been developing rapidly and has resulted in breakthroughs for the treatment of various diseases.
Treatments utilizing stems cells often require stem cells to be exposed to inflammatory environments, such as vascular cell adhesion molecule-1, tumor necrosis factor α (TNFα), and interleukin 6 (IL6). Stem cell-derived exosomes are especially important in producing miRNAs that impact angiogenesis.
MicroRNAs (miRNAs) are RNAs 0-20 nucleotides in length, which are derived from hairpin-like precursor miRNAs. They acts as important regulators of mRNA expression. It has been reported that miRNAs play critical roles in some cells and have the potential as diagnostic and therapeutic biomarkers.
The morphology and quantity of mesenchymal stem cell (MSC) exosomes (MSCs-exo) are influenced by different inflammatory cytokine environments.
The morphology and quantity of each group of MSC exosomes were observed and measured. The miRNAs in MSCs-exo were sequenced. Differential expression of miRNAs and their target genes as well as the related regulatory mechanisms were researched.
TNFα and IL6 may influence the expression of miRNAs that down-regulate the PI3K-AKT, MAPK, and VEGF signaling pathways; particularly, IL6 significantly down-regulates the PI3K-AKT signaling pathway.
Overall, inflammatory cytokines may lead to changes in exosomal miRNAs that abnormally impact cellular components, molecular function, and biological process, particularly angiogenesis.