Sasikala M, Surya P, Radhika G, Kumar PP, Rao MS, Mukherjee RM, Rao PN, Reddy DN. Identification of circulating CD90+ CD73+ cells in cirrhosis of liver. World J Stem Cells 2011; 3(7): 63-69 [PMID: 21860671 DOI: 10.4252/wjsc.v3.i7.63]
Corresponding Author of This Article
Mitnala Sasikala, PhD, Senior Scientist, Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, 6-3-661, Somajiguda, Hyderabad 500082, India. aigres.mit@gmail.com
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Mitnala Sasikala, Pugazhelthi Surya, Gaddipati Radhika, Pondugala Pavan Kumar, Mekala Subba Rao, Rathindra Mohan Mukherjee, Institute of Basic Sciences and Translational Research, Asian Health Care Foundation, Hyderabad 500082, India
Padaki Nagaraja Rao, D Nageshwar Reddy, Asian Institute of Gastroenterology, Hyderabad 500082, India
Author contributions: Sasikala M designed the study, analyzed data and wrote the manuscript; Surya P, Radhika G and Pavan Kumar P performed the majority of experiments; Rao MS and Mukherjee RM were involved in editing the manuscript; Rao PN and Reddy DN co-ordinated and were involved in selection of patients and final approval of the manuscript.
Supported by Asian Health Care Foundation
Correspondence to: Mitnala Sasikala, PhD, Senior Scientist, Institute of Basic Sciences and Translational Research, Asian Healthcare Foundation, 6-3-661, Somajiguda, Hyderabad 500082, India. aigres.mit@gmail.com
Received: October 9, 2010 Revised: January 15, 2011 Accepted: January 25, 2011 Published online: July 26, 2011
Abstract
AIM: To identify circulating CD90+ CD73+ CD45- cells and evaluate their in vitro proliferating abilities.
METHODS: Patients with cirrhosis (n = 43), and healthy volunteers (n = 40) were recruited to the study. Mononuclear cells were isolated and cultured from the peripheral blood of controls and cirrhosis patients. Fibroblast-like cells that appeared in cultures were analyzed for morphological features, enumerated by flow cytometry and confirmed by immunocytochemistry (ICC). Colony forming efficiency (CFE) of these cells was assessed and expressed as a percentage.
RESULTS: In comparison to healthy volunteers, cells obtained from cirrhotic patients showed a significant increase (P < 0.001) in the percentage of CD90+ CD73+ CD45- cells in culture. Cultured cells also showed 10 fold increases in CFE. Flow cytometry and ICC confirmed that the proliferating cells expressed CD90+ CD73+ in the cultures from cirrhosis patients.
CONCLUSION: These results indicate the presence of circulating CD90+ CD73+ CD45- cells in patients with liver cirrhosis that have the potential to proliferate at a higher rate.
