Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Dec 26, 2020; 12(12): 1640-1651
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1640
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
Cintia DS Horinouchi, María Julia Barisón, Anny W Robert, Crisciele Kuligovski, Alessandra M Aguiar, Bruno Dallagiovanna
Cintia DS Horinouchi, María Julia Barisón, Anny W Robert, Crisciele Kuligovski, Alessandra M Aguiar, Bruno Dallagiovanna, Stem Cells Basic Biology Laboratory, Instituto Carlos Chagas, Curitiba 81350010, Paraná, Brazil
Author contributions: Horinouchi CDS, Barisón MJ and Robert AW contributed equally to this work; Horinouchi CDS, Barisón MJ, Robert AW, de Aguiar AM and Dallagiovanna B designed and coordinated the study; Horinouchi CDS, Barisón MJ, Robert AW and Kuligovski C performed the experiments and acquired and analyzed data; Horinouchi CDS, Barisón MJ, Robert AW, Kuligovski C, de Aguiar AM and Dallagiovanna B interpreted the data; Horinouchi CDS, Barisón MJ and Robert AW wrote the manuscript; de Aguiar AM and Dallagiovanna B critically reviewed the manuscript; and all authors approved the final version of the article.
Supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico, No. 442353/2019-7 and No. 442375/2019-0.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at Fundação Oswaldo Cruz.
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Bruno Dallagiovanna, PhD, Research Scientist, Stem Cells Basic Biology Laboratory, Instituto Carlos Chagas, Rua Professor Algacyr Munhoz Mader, 3775, Curitiba 81350010, Paraná, Brazil. bruno.dallagiovanna@fiocruz.br
Received: July 16, 2020
Peer-review started: July 16, 2020
First decision: August 9, 2020
Revised: October 9, 2020
Accepted: November 5, 2020
Article in press: November 5, 2020
Published online: December 26, 2020
Processing time: 163 Days and 8.5 Hours
Abstract
BACKGROUND

Human adipose-derived stromal/stem cells (hASCs) are one of the most useful types of mesenchymal stromal/stem cells, which are adult multipotent cells with great therapeutic potential for the treatment of several diseases. However, for successful clinical application, it is critical that high-quality cells can be obtained. Diverse factors seem to be able to influence cell quality and performance, especially factors related to donors’ intrinsic characteristics, such as age. Nevertheless, there is no consensus regarding this characteristic, and there is conflicting information in the literature.

AIM

To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.

METHODS

hASCs were harvested from liposuctioned adipose tissue obtained from female donors (aged 20-70 years). Cells were distributed into two groups according to age range: old hASCs (oASCs, ≥ 55 years, n = 9) and young hASCs (yASCs, ≤ 35 years, n = 9). For each group, immunophenotypic characterization was performed by flow cytometry. Population doubling time was assessed over seven days. For adipogenic potential evaluation, lipid deposits were assessed after 7 d, 14 d and 21 d of adipogenic induction. Osteogenic potential was verified by analyzing cell mineralization after 14 d, 21 d and 28 d of osteogenic induction. mRNA expression of PPARγ2, CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.

RESULTS

hASCs were successfully obtained, cultured, and grouped according to their age: yASCs (26.33 ± 4.66 years old) and oASCs (64.78 ± 4.58 years old). After maintenance of the cells in culture, there were no differences in morphology between cells from the young and old donors. Additionally, both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells. The average doubling time indicated that yASCs (4.09 ± 0.94 d) did not significantly differ from oASCs (4.19 ± 1.29 d). Concerning differentiation potential, after adipogenic and osteogenic induction, yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells. However, no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis. Additionally, the mRNA expression of PPARγ2, CEBPA and Runx2 were similar in yASCs and oASCs.

CONCLUSION

Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates.

Keywords: Adipose-derived stem cells; Stem cells; Adipogenesis; Osteogenesis; Cell differentiation; Donor age

Core Tip: Adipose-derived stem cells are adult multipotent stem cells with great therapeutic potential for a variety of diseases. The donor’s intrinsic characteristics, such as sex, health conditions and age, have been claimed to be the main factors responsible for affecting the quality and the performance of these cells. Regarding donor age, the literature shows many conflicting observations. Here, we evaluated the proliferation and differentiation potential of adipose-derived stem cells isolated from lipoaspirates obtained from elderly and young female donors. Our results demonstrated that age does not seem to significantly affect the cell division or adipogenic or osteogenic potential of these cells.