Liu W, Deng C, Godoy-Parejo C, Zhang Y, Chen G. Developments in cell culture systems for human pluripotent stem cells. World J Stem Cells 2019; 11(11): 968-981 [PMID: 31768223 DOI: 10.4252/wjsc.v11.i11.968]
Corresponding Author of This Article
Guokai Chen, PhD, Associate Professor, Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, E12-4013, Taipa, Macau 999078, China. guokaichen@umac.mo
Research Domain of This Article
Cell Biology
Article-Type of This Article
Review
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Stem Cells. Nov 26, 2019; 11(11): 968-981 Published online Nov 26, 2019. doi: 10.4252/wjsc.v11.i11.968
Developments in cell culture systems for human pluripotent stem cells
Weiwei Liu, Chunhao Deng, Carlos Godoy-Parejo, Yumeng Zhang, Guokai Chen
Weiwei Liu, Chunhao Deng, Carlos Godoy-Parejo, Yumeng Zhang, Guokai Chen, Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau 999078, China
Weiwei Liu, Chunhao Deng, Carlos Godoy-Parejo, Yumeng Zhang, Guokai Chen, Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau 999078, China
Weiwei Liu, Bioimaging and Stem Cell Core Facility, Faculty of Health Sciences, University of Macau, Macau 999078, China
Author contributions: Liu W, Deng C, Godoy-Parejo C, Zhang Y, and Chen G wrote the manuscript; Deng C drew the figure; Godoy-Parejo C compiled the tables.
Supported byUniversity of Macau Multi-Year Research Grants, No. MYRG2015-00228-FHS and MYRG2018-00135-FHS; and Macao Science and Technology Development Fund, No. FDCT/131/2014/A3 and FDCT/056/2015/A2.
Conflict-of-interest statement: Dr. Chen has a patent 9644186 with royalties paid to Wisconsin Alumni Research Foundation. The other authors have no conflict of interest to declare.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Guokai Chen, PhD, Associate Professor, Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, E12-4013, Taipa, Macau 999078, China. guokaichen@umac.mo
Telephone: +853-88224985
Received: March 26, 2019 Peer-review started: March 28, 2019 First decision: April 16, 2019 Revised: August 21, 2019 Accepted: August 27, 2019 Article in press: August 27, 2019 Published online: November 26, 2019 Processing time: 225 Days and 0.8 Hours
Abstract
Human pluripotent stem cells (hPSCs) are important resources for cell-based therapies and pharmaceutical applications. In order to realize the potential of hPSCs, it is critical to develop suitable technologies required for specific applications. Most hPSC technologies depend on cell culture, and are critically influenced by culture medium composition, extracellular matrices, handling methods, and culture platforms. This review summarizes the major technological advances in hPSC culture, and highlights the opportunities and challenges in future therapeutic applications.
Core tip: This review summarizes recent developments in cell culture systems for human pluripotent stem cells, including signal transduction requirements at different pluripotency stages, advances in extracellular matrices and handling methods, establishment of chemically defined conditions, and various cell culture platforms for specific purposes.
