Lack of inhibitory effects of Lactic acid bacteria on 1,2-dimethylhydrazine-induced colon tumors in rats

Table 1 Average final body weight, relative liver and kidney weights, and food consumption data (22wk)

Group	Treatment	No. of rats	Final body Wt, (g)	Relative liverWt, (g)	Relative kidneyWt, (g)	Average food consumption (g/rat/day)
1	DMH + lactic acid	10	389.6 ± 44.1e	2.92 ± 0.24	0.54 ± 0.06	19.45
2	Saline + vehicle	10	439.5 ± 39.3	3.09 ± 0.35	0.56 ± 0.12	20.07
3	DMH	10	383.5 ± 19.2e	3.10 ± 0.40	0.55 ± 0.07	18.48

a: Values are means ± SD, DMH, 1,2-dimethylhydrazine, b: Statistical significance:

^eP < 0.05, Student’s t-test, vs Group 2. c: Kidney weight values are totals for both kidneys.

Table 2 Colon tumor incidence, classification, multiplicity, tumor volume and stage in rats treated with DMH followed or not by lactic acid bacteria (22wk)^a

Treatment	Incidencen (%)	Total no.tumors	Adenoma No (%)	Carcinoma in situ No (%)	Carcinoma No (%)	Multiplicityb No.	Tumor volumemm3	Duke’s stagec
								A	B	C
DMH + lactic acid	9 (90)	28	12 (43)	8 (28.5)	8 (28.5)	3.11 ± 2.00	1.64 ± 1.92	0	2	1
DMH	10 (100)	40	27 (67.5)	3 (7.5)	10 (25)	4.00 ± 2.96	4.31 ± 4.56	0	0	3

a: Values are means ± SD. b: No. of tumors/tumor-bearing rat. c: No. of rats with carcinoma were allocated to one of three tumor stages.