Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain

Table 1 ³H-TdR incorporation quantity (¯x ± s CPM/Well)

Group	2nd day	3rd day	4th day
Ad-p27kip1	2013.42 ± 84.38a	1546.54 ± 104.14a	244.22 ± 39.62a
Ad-LacZ	3316.81 ± 96.37b	3574.21 ± 68.58b	4451.38 ± 154.67b
Blank	3564.34 ± 72.82	3685.72 ± 122.23	4468.65 ± 237.36

^aP < 0.001 vs Blank group,

^bP > 0.05 Ad-LacZ group vs Blank group.

Table 2 Incorporation quantity of ³H-Leucine (¯x ± s CPM/Well)

Group	2nd day	3rd day	4th day
Ad-p27kip1	595.16 ± 57.06a	422.82 ± 45.81a	267.58 ± 37.56a
Ad-LacZ	886.47 ± 48.47b	915.44 ± 93.58b	1139.46 ± 89.24b
Blank	895.21 ± 967.52	967.52 ± 78.35	1243.95 ± 06.72

^aP < 0.001 vs Blank group,

^bP > 0.05 Ad-LacZ group vs Blank group.

Table 3 Effect of Ad-p27 on EC9706 cell cycle through fluid cell meter

Group	Distribution of cell cycle (%)
	G0/G1	S	G2/M
Blank	40.39 ± 3.86	49.61 ± 4.27	13.10 ± 1.03
Ad-LacZ	44.17 ± 2.63	39.56 ± 3.39	16.27 ± 2.85
Ad-p27kip1	66.52 ± 5.48ab	21.15 ± 3.26ab	12.33 ± 2.72

^aP < 0.05 vs Blank group,

^bP < 0.05 vs Ad-LacZ group.