Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain

doi:10.3748/wjg.v9.i11.2404

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Esophageal Cancer

World J Gastroenterol. Nov 15, 2003; 9(11): 2404-2408
Published online Nov 15, 2003. doi: 10.3748/wjg.v9.i11.2404

Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain

Qing-Ming Wu, Jie-Ping Yu, Qiang Tong, Xiao-Hu Wang, Guo-Jian Xie

Qing-Ming Wu, Jie-Ping Yu, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

Qiang Tong, Xiao-Hu Wang, Guo-Jian Xie, Department of Gastroenterology, Taihe Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Qing-Ming Wu, Department of Gastroenterology, Taihe Hospital of Yunyang Medical College, 29 Renmin Road, Shiyan 442000, Hubei Province, China. wuhe9224@sina.com

Telephone: +86-719-8801431 Fax: +86-719-8883809

Received: March 4, 2003
Revised: March 24, 2003
Accepted: June 2, 2003
Published online: November 15, 2003

Abstract

AIM: To investigate the inhibition of p27kip1 gene on the growth of esophageal carcinoma cell strain (EC9706).

METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effect on p27kip1 expression, the growth of esophageal carcinoma cell, DNA replication, protein synthesis, cell multiplication and apoptosis were explored by means of cell growth count, ³H-TdR, ³H-Leucine incorporation, flow cytometry, DNA fragment analysis and TUNEL.

RESULTS: Recombinant adenovirus Ad-p27kip1 was successfully constructed with a virus titer of 1.24 × 10¹² pfu/mL. p27kip protein expression increased markedly after EC-9706 transfection, while incorporation quantity of ³H-TdR and ³H-Leucine decreased significantly. The growth of esophageal carcinoma cell was inhibited obviously. Testing of flow cytometry displayed a typical apoptosis peak, and DNA gel electrophoresis showed a typical apoptosis ladder. TUNEL showed the apoptosis rate of Ad-p27kip1 group and control group to be 37.3% and 1.26% (P < 0.001) respectively.

CONCLUSION: Ad-p27kip1 can inhibit the growth and multiplication of esophageal carcinoma cells and induce apoptosis. Therefore, enhanced p27kip1 expression may be a new way to treat esophageal carcinoma.

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