Interaction between cisplatin, 5-fluorouracil and vincristine on human hepatoma cell line (7721)

Table 1 Dose-effect relationships of three drugs used alone and in combination

Cis	5-Fu	VCR	Cis + 5-Fu	VCR + 5-Fu	Cis + VCR
(2.5) 0.662	(25) 0.600	(0.25) 0.178	(2.5 + 25) 0.709	(2.5 + 25) 0.647	(2.5 + 25) 0.527
(5.0) 0.809	(50) 0.784	(0.50) 0.204	(5 + 50) 0.859	(5 + 50) 0.713	(5 + 50) 0.781
(10.0) 0.867	(100) 0.834	(1.00) 0.282	(10 + 100) 0.877	(10 + 100) 0.810	(10 + 100) 0.844
(20.0) 0.893	(200) 0.853	(2.00) 0.429	(20 + 200) 0.902	(20 + 200) 0.839	(20 + 200) 0.873
(40.0) 0.928	(400) 0.871	(4.00) 0.446	(40 + 400) 0.951	(40 + 400) 0.892	(40 + 400) 0.898

Table 2 Fa and CI of drug combinations at different ratios

Drug 1 (mg/L)		Drug 2 (mg/L)		fa	CI	Drug 1 (mg/L)		Drug 2 (mg/L)		fa	CI
Cis	2.5	5-Fu	100	0.821	0.75	Cis	2.5	VCR	2	0.554	1.15
	5		100	0.856	0.85		5		2	0.858	0.80
	10		100	0.877	0.87		10		2	0.862	0.74
	20		100	0.883	1.09		20		2	0.873	0.71
	40		100	0.893	1.23		40		2	0.904	0.66
Cis	2.5	5-Fu	200	0.839	1.85	VCR	0.25	5-Fu	100	0.657	2.39
	5		200	0.867	2.93		0.5		100	0.761	1.15
	10		200	0.899	2.95		1		100	0.810	0.77
	20		200	0.902	3.00		2		100	0.826	0.64
	40		200	0.907	3.36		4		100	0.850	0.51
Cis	2.5	VCR	1	0.498	0.32	VCR	0.25	5-Fu	200	0.759	2.13
	5		1	0.825	1.38		0.5		200	0.783	1.79
	10		1	0.844	2.05		1		200	0.825	1.28
	20		1	0.852	2.09		2		200	0.839	1.10
	40		1	0.894	3.40		4		200	0.867	0.92

Table 3 Relationship between time-order and effect in drugs used in combination (^-x±s)

Drugs (mg/L)		Simultaneous exposure	Pre-Cis exposure	Post-Cis exposure
Cis + 5-Fu
2.5	25	0.709 ± 0.015	0.683 ± 0.016	0.598 ± 0.010
5	50	0.859 ± 0.011	0.807 ± 0.027	0.773 ± 0.015
10	100	0.877 ± 0.013	0.824 ± 0.012	0.793 ± 0.026
20	200	0.902 ± 0.014	0.831 ± 0.017	0.805 ± 0.016
40	400	0.951 ± 0.012	0.853 ± 0.015	0.822 ± 0.010
Cis + VCR
2.5	0.25	0.527 ± 0.025	0.470 ± 0.023	0.199 ± 0.023
5	0.5	0.781 ± 0.034	0.707 ± 0.022	0.407 ± 0.017
10	1	0.844 ± 0.015	0.789 ± 0.025	0.533 ± 0.013
20	2	0.873 ± 0.017	0.818 ± 0.029	0.651 ± 0.021
40	4	0.898 ± 0.025	0.893 ± 0.020	0.752 ± 0.025