Partial isolation and identification of hepatic stimulator substance mRNA extracted from human fetal liver

Table 1 Effect of translated products directed by different mRNA fractions on DNA synthesis of SMMC-7721 hepatoma cells

Fractions	cpm	SI
Control	2450 ± 452
Purified hHSS	5384 ± 602	2.1a
Unfractionated mRNA	2430 ± 292	1.0
Fraction 6	2202 ± 389	0.9
Fraction 8	5013 ± 382	2.0a
Fraction 9	4123 ± 452	1.7a
Fraction 10	1894 ± 172	0.7

Data are presented as x ± s and based on the results of four experiments (four samples for each).

^aP < 0.05, showing stimulating effect.

Table 2 Organ specificity of translated products of specific mRNA (x^-± s)

Test system	Control	Translated product of specific mRNA	Purified hHSS
SMMC-7721	1245 ± 274	4703 ± 236a	4279 ± 193a
HFL	346 ± 79	758 ± 102a	693 ± 87a
HFK	308 ± 74	298 ± 70	378 ± 92
HFS	248 ± 63	304 ± 74	238 ± 75
K562	2438 ± 256	2058 ± 217	3072 ± 287
HL-60	4308 ± 362	4703 ± 298	3994 ± 304

HFL, human fetal liver primary culture; HFK, human fetal kid-ney primary culture; HFS, human fetal spleen primary culture; hHSS, purified human hepatic stimulation substance.

^aP < 0.05, showing stimulating effect.

Table 3 Heat resistance of translated products of specific mRNA (x^-± s)

Treatment	Translated product of specific mRNA (fraction 8, 9)		Purified hHSS
	cpm	SI	cpm	SI
Control	2034 ± 148		1876 ± 138
NO heating	4703 ± 236	2.3	4279 ± 193	2.2
65 °C for 10 min	4306 ± 287	2.1	5268 ± 294	2.9
95 °C for 10 min	4579 ± 263	2.2	5438 ± 306	3.0

Data are based on the results of three experiments (four samples for each). The target cell was SMMC-7721 hepatoma cell.