Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 1998; 4(2): 100-102
Published online Apr 15, 1998. doi: 10.3748/wjg.v4.i2.100
Partial isolation and identification of hepatic stimulator substance mRNA extracted from human fetal liver
Xiao-Ming Yang, Ling Xie, Gui-Chun Xing, Zu-Ze Wu, Fu-Chu He
Xiao-Ming Yang, Ling Xie, Gui-Chun Xing, Zu-Ze Wu, Fu-Chu He, Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, China
Xiao-Ming Yang, male, born on 1962-12-08 in Shaoyang City, Hunan Province, Associate Professor of Molecular and Cell Biology, having 24 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Fu-Chu He, Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, China
Telephone: +86·10·66931246
Received: July 10, 1997
Revised: August 21, 1997
Accepted: September 10, 1997
Published online: April 15, 1998
Abstract

AIM: To partially isolate and identify hepatic stimulator substance mRNA from human fetal liver tissues.

METHODS: The poly (A) mRNA was extracted from human fetal liver tissues of 4-5 month gestation, fractionated by size on sucrose gradient centrifugation, translated into protein from each fraction in vitro and then its products were tested for HSS activity.

RESULTS: Twenty-two 500 μg total RNA was obtained from human fetal liver tissues and pooled. mRNA of 420 μg was yielded, processed by oligo (dT)-cellulose column chromatography, then was size-fractionated by ultracentrifution on a continuous sucrose density gradient (5%-25%), and separated into 18 fractions. Translated products of mRNA in fraction 8 and 9 could produce a two-fold increase in the incorporation of 3H-TdR into DNA of SMMC-7721 hepatoma cells and in a heat resistant and organ-specific way.

CONCLUSION: The partially purified HSS mRNA was obtained and this would facilitate the cloning of HSS using expression vectors.

Keywords: fetal liver tissue, RNA, messenger, hepatic stimulator substance, hepatocyte proliferation