Clinical effects of phospholipase D2 in attenuating acute pancreatitis

Table 1 General statistics, n (%)

Parameter	Mild group (n = 25)	Moderate group (n = 30)	Severe group (n = 35)	Variance ratio	P value
Gender (male:female)	Eleven past two p.m.	Thirteen past five p.m.	Thirteen past ten p.m.	3.109	0.413
Age (years)	45.17 ± 4.28	46.83 ± 4.66	45.92 ± 5.18	5.226	0.385
BMI (kg/m2)	22.46 ± 1.75	23.56 ± 1.49	22.85 ± 1.88	4.035	0.307
Smoker	8 (32.00)	10 (33.33)	12 (34.28)	2.461	0.216
Alcohol consumption	6 (24.00)	8 (26.67)	11 (31.42)	1.975	0.445

Table 2 Protein expression analysis of phospholipase D2

Group	PLD2
Mild group (n = 25)	1.49 ± 0.10
Moderate group (n = 30)	1.03 ± 0.05
Severe group (n = 35)	0.54 ± 0.02
Variance ratio	13.226
P value	0.015

PLD2: Phospholipase D2.

Table 3 The mRNA expression analysis of neutrophil migration-related chemokine receptor

Group	CXCR1	CXCR2	CCR2	CCR5
Mild group (n = 25)	1.02 ± 0.01	0.97 ± 0.01	1.06 ± 0.02	1.01 ± 0.01
Moderate group (n = 30)	2.28 ± 0.07	2.05 ± 0.03	1.98 ± 0.06	1.49 ± 0.05
Severe group (n = 35)	4.16 ± 0.16	4.94 ± 0.15	3.02 ± 0.13	1.98 ± 0.17
Variance ratio	9.336	12.208	13.115	10.624
P value	0.002	0.014	0.005	0.006

CXCR1: C-X-C chemokine receptor type 1; CXCR2: C-X-C chemokine receptor type 2; CCR2: C-C chemokine receptor type 2; CCR5: C-C chemokine receptor type 5.

Table 4 Enzyme-linked immunosorbent assay detection of inflammatory factors

Group	CRP (mg/L)	TNF-α (pg/mL)	IL-1β (pg/mL)	IL-6 (pg/mL)
Mild group (n = 25)	75.68 ± 10.43	36.45 ± 8.24	14.39 ± 3.18	135.88 ± 15.05
Moderate group (n = 30)	136.29 ± 10.67	68.24 ± 10.67	36.27 ± 6.33	536.72 ± 25.46
Severe group (n = 35)	194.18 ± 17.34	105.67 ± 12.49	58.41 ± 8.62	768.55 ± 36.19
Variance ratio	15.389	11.663	9.419	12.056
P value	0.015	0.026	0.003	0.006

CRP: C-reactive protein; TNF: Tumor necrosis factor; IL: Interleukin.

Table 5 In vitro migration test of neutrophils

Group	Number of neutrophil migration (n)
Mild group (n = 25)	235.62 ± 23.15
Moderate group (n = 30)	568.39 ± 49.37
Severe group (n = 35)	856.27 ± 54.28
Variance ratio	13.226
P value	0.015

Table 6 In vitro migration test of neutrophils

Group	Number of neutrophil migration (n)
Mild group (n = 25)	212.24 ± 24.66
Mild group + PLD2 inhibitor group (n = 25)	473.69 ± 35.17
Moderate group (n = 30)	536.19 ± 44.58
Moderate group + PLD2 inhibitor group (n = 30)	755.03 ± 50.19
Severe group (n = 35)	828.61 ± 58.21
Severe group + PLD2 inhibitor group (n = 35)	1126.75 ± 64.38
Variance ratio	16.204
P value	0.001

PLD2: Phospholipase D2.