Niu JW, Zhang GC, Ning W, Liu HB, Yang H, Li CF. Clinical effects of phospholipase D2 in attenuating acute pancreatitis. World J Gastroenterol 2025; 31(2): 97239 [DOI: 10.3748/wjg.v31.i2.97239]
Corresponding Author of This Article
Chao-Feng Li, MD, Department of General Surgery, China-Japan Friendship Hospital, No. 2 Yinghuayuan Dongjie, Beijing 100029, China. 13488880631@139.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Retrospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Jin-Wei Niu, Guo-Chao Zhang, Wu Ning, Hai-Bin Liu, Hua Yang, Chao-Feng Li, Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China
Author contributions: Niu JW and Li CF contributed to the conception and design of the study; Niu JW, Zhang GC, Ning W, Liu HB, Yang H, and Li CF participated in the clinical practice, including diagnosis, treatment, consultation and follow up of patients; Zhang GC and Ning W contributed to the acquisition of data; Liu HB and Yang H contributed to the analysis of data; Niu JW wrote the manuscript; Li CF revised the manuscript. All authors approved the final version of the manuscript.
Institutional review board statement: The study protocol was approved by the Ethics Committee of China-Japan Friendship Hospital, approval No. 23-B045, and the study was performed in accordance with the Helsinki II declaration.
Informed consent statement: Informed consent was obtained from all the study subjects before enrollment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Data is provided within the manuscript or supplementary information files.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Chao-Feng Li, MD, Department of General Surgery, China-Japan Friendship Hospital, No. 2 Yinghuayuan Dongjie, Beijing 100029, China. 13488880631@139.com
Received: May 26, 2024 Revised: October 8, 2024 Accepted: November 26, 2024 Published online: January 14, 2025 Processing time: 205 Days and 17.7 Hours
Abstract
BACKGROUND
The objective of the current study was to elucidate the clinical mechanism through which phospholipase D2 (PLD2) exerted a regulatory effect on neutrophil migration, thereby alleviating the progression of acute pancreatitis.
AIM
To elucidate the clinical mechanism through which PLD2 exerted a regulatory effect on neutrophil migration, thereby alleviating the progression of acute pancreatitis.
METHODS
The study involved 90 patients diagnosed with acute pancreatitis, admitted to our hospital between March 2020 and November 2022. A retrospective analysis was conducted, categorizing patients based on Ranson score severity into mild (n = 25), moderate (n = 30), and severe (n = 35) groups. Relevant data was collected for each group. Western blot analysis assessed PLD2 protein expression in patient serum. Real-time reverse transcription polymerase chain reaction was used to evaluate the mRNA expression of chemokine receptors associated with neutrophil migration. Serum levels of inflammatory factors in patients were detected using enzyme-linked immunosorbent assay. Transwell migration tests were conducted to compare migration of neutrophils across groups and analyze the influence of PLD2 on neutrophil migration.
RESULTS
Overall data analysis did not find significant differences between patient groups (P > 0.05). The expression of PLD2 protein in the severe group was lower than that in the moderate and mild groups (P < 0.05). The expression level of PLD2 in the moderate group was also lower than that in the mild group (P < 0.05). The severity of acute pancreatitis is negatively correlated with PLD2 expression (r = -0.75, P = 0.002). The mRNA levels of C-X-C chemokine receptor type 1, C-X-C chemokine receptor type 2, C-C chemokine receptor type 2, and C-C chemokine receptor type 5 in the severe group are significantly higher than those in the moderate and mild groups (P < 0.05), and the expression levels in the moderate group are also higher than those in the mild group (P < 0.05). The levels of C-reactive protein, tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the severe group were higher than those in the moderate and mild groups (P < 0.05), and the levels in the moderate group were also higher than those in the mild group (P < 0.05). The number of migrating neutrophils in the severe group was higher than that in the moderate and mild groups (P < 0.05), and the moderate group was also higher than the mild group (P < 0.05). In addition, the number of migrating neutrophils in the mild group combined with PLD2 inhibitor was higher than that in the mild group (P < 0.05), and the number of migrating neutrophils in the moderate group combined with PLD2 inhibitor was higher than that in the moderate group (P < 0.05). The number of migrating neutrophils in the severe group + PLD2 inhibitor group was significantly higher than that in the severe group (P < 0.05), indicating that PLD2 inhibitors significantly stimulated neutrophil migration.
CONCLUSION
PLD2 exerted a crucial regulatory role in the pathological progression of acute pancreatitis. Its protein expression varied among patients based on the severity of the disease, and a negative correlation existed between PLD2 expression and disease severity. Additionally, PLD2 appeared to impede acute pancreatitis progression by limiting neutrophil migration.
Core Tip: This research substantiated the indispensable role of phospholipase D2 (PLD2) in the pathological progression of acute pancreatitis. Moreover, the varying protein expression levels of PLD2 among patients with distinct degrees of acute pancreatitis exhibited an inverse relationship with disease severity. Moreover, this study revealed PLD2’s potential to mitigate acute pancreatitis by restricting neutrophil movement.
