Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

Table 1 The effects of dimethyl sulfate concentration on the survival and the rate of attachment of SMMC-7721 cells after cryopreservation at -50 °C

5	5	10	10	15	15	20	20	30	30	40	40	DMSO (%)
0	10	0	10	0	10	0	10	0	10	0	10	FCS (%)
90 ± 2	90 ± 3	95 ± 2	98 ± 2	98 ± 1	99 ± 1	93 ± 4	95 ± 2	87 ± 4	98 ± 2	80 ± 4	83 ± 2	SR (%)
60 ± 3	65 ± 2	85 ± 4	90 ± 3	85 ± 3	90 ± 2	55 ± 2	60 ± 4	20 ± 4	25 ± 5	1 ± 1	2 ± 1	AR (%)

SR: Survival rate; AR: Attaching rate.

Table 2 The effects of -50 °C cryopreservation on the activity of lactate dehydrogenase (%) of human hepatoma SMMC-7721 cell line

	LDH5	LDH4	LDH3	LDH2	LDH1
SC (AC)	28.3	48.3	18.9	4.4	0
SC (E)	14.9	26.8	29.3	25.3	3.6
BEL7404 cell	1.7	22.95	37.76	27.21	10.38

SC (AC): SMMC-7721 cell after cryopreservation; SC (E): SMMC-7721 cell in the establishment; BEL7404 cell: Another human hepatoma cell line.