Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 &deg;C

doi:10.3748/wjg.v3.i3.150

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 3, Issue 3

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (1955)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series, Tables (1-2) series.

Item

Count

PDF

234

HTML

1438

Figures (1-2)

143

Tables (1-2)

140

Sum=1955

Sep 15, 1997 (publication date) through Dec 23, 2024

Times Cited of This Article

Times Cited (0)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Research

World J Gastroenterol. Sep 15, 1997; 3(3): 150-152
Published online Sep 15, 1997. doi: 10.3748/wjg.v3.i3.150

Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

Shi-Ming Jiang, Zhao-Hui Xu, Yan Zhang, Xian-Min Shi

Shi-Ming Jiang, Zhao-Hui Xu, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China

Yan Zhang, Shanghai Institute of Cell Biology, Academia Sinica

Xian-Min Shi, Biology Department, Xiamen University

Author contributions: All authors contributed equally to the work.

Supported by a grant from Shandong Provincial Science and Technology Committe, No. K91236517.

Correspondence to: Dr. Shi-Ming Jiang, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China

Received: October 26, 1996
Revised: December 19, 1996
Accepted: January 22, 1997
Published online: September 15, 1997

Abstract

AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.

METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.

RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.

CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.

Keywords: Liver neoplasms; Carcinoma, hepatocellular; Cryopreservation; Tumor cells, cultured; Phenotype