Qingyi decoction protects against myocardial injuries induced by severe acute pancreatitis

Table 1 List of predesigned primers

No	Primer	Sequence
1	GAPDH-F	5’-TGTGTCCGTCGTGGATCTGA-3’
2	GAPDH-R	5’-TTGCTGTTGAAGTCGCAGGAG-3
3	STIM1-F	5’-TCCACCTGGAGAAGAAGCTG-3
4	STIM1-R	5’-CCTCAGGAGCATACCAGGAG-3’
5	Orai1-SOCE-F	5’-CGCCCTCATGATCAGTACCT-3’
6	Orai1-SOCE-R	5’-AGAACTTCACCCAGCAGAGC-3

Table 2 Morbidity and mortality rates 24 h after severe acute pancreatitis induction

Group	Number	Morbidity	%	Mortality	%	P value
Sham	10	0	0	0	0	-
SAP	10	10	100	4	40	< 0.0001 (Sham vs SAP)
QYD	10	10	100	1	10	0.0047 (SAP vs QYD)
2-APB	10	10	100	1	10	NS (QYD vs 2-APBSAP)

SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate.

Table 3 Effect of Qingyi decoction treatment on serum cytokine levels

Group	Case (n)	IL-1β	IL-6	IL-8	IL-12	AMY	TNF-α
Sham	10	09.2 ± 1.8	61.7 ± 4.0	109.8 ± 6.0	062 ± 6.0	076 ± 3.0	121 ± 4.0
SAP	10	17.3 ± 2.2	93.0 ± 5.0	116.8 ± 4.0	099 ± 5.0	154 ± 6.0	354 ± 6.0
2-APB	10	11.0 ± 09	76.0 ± 8.0	128.0 ± 9.0	076 ± 7.0	102 ± 2.0	163 ± 9.0
QYD	10	11.9 ± 1.7	69.0 ± 6.0	132.0 ± 2.0	073 ± 4.0	114 ± 4.0	161 ± 7.0

Data are expressed as mean ± SE; P < 0.05. SAP: Severe acute pancreatitis; QYD: Qingyi decoction; AMY: Amyloid β; TNF-α: Tumor necrosis factor alpha; 2-APB: 2-Aminoethyl diphenylborinate.

Table 4 Apoptotic index among the different groups

Group	Cases (n)	Apoptotic %	P value
Sham	10	1.5 ± 0.3	-
SAP	6	38.8 ± 2.7	< 0.0001 (Sham vs SAP)
2-APB	9	15.4 ± 1.9	< 0.0001 (SAP vs 2-APB)
QYD	9	13.7 ± 1.4	< 0.0001 (SAP vs QYD)

Results are expressed as mean ± SD; P < 0.05. SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate.