Copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Qingyi decoction protects against myocardial injuries induced by severe acute pancreatitis
Lei Li, Yong-Qi Li, Zhong-Wei Sun, Cai-Ming Xu, Jun Wu, Ge-Liang Liu, Ahmed MH Bakheet, Hai-Long Chen
Lei Li, Department of Vascular Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China
Lei Li, Zhong-Wei Sun, Cai-Ming Xu, Hai-Long Chen, Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
Yong-Qi Li, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 3058575, Japan
Zhong-Wei Sun, Department of Emergency Medicine, The Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
Cai-Ming Xu, Department of Traditional Chinese Medicine, Dalian Obstetrics and Gynecology Hospital, Dalian 116021, Liaoning Province, China
Jun Wu, Department of Ultrasound, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China
Ge-Liang Liu, Department of Urology Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China
Ahmed MH Bakheet, Department of Pathology, The Third Affiliated Hospital of San Yet-sen University, Guangzhou 510360, Guangdong Province, China
Author contributions: Li YQ and Li L contributed equally to this work. Li L, Li YQ, and Chen HL designed the research; Li L, Li YQ, Sun ZW, and Xu CM performed the research; Wu J and Liu GL contributed new reagents; Xu CM, Wu J, and Bakheet AMH analyzed the data; Li L and Li YQ wrote the paper.
Supported by the National Natural Science Foundation of China, No, 81573751.
Institutional animal care and use committee statement: Forty adult healthy male Sprague-Dawley rats, weighing between 180-220 g, were obtained from the Specific Pathogen Free Animal Department of Dalian Medical University (Dalian, China), and the study protocol followed the ethical guidelines approved by the Dalian Medical University Animal Care and Use Committee.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Hai-Long Chen, MD, PhD, Chief Doctor, Department of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian 116011, Liaoning Province, China. hailongchen2018@163.com
Received: December 6, 2019
Peer-review started: December 6, 2019
First decision: January 16, 2020
Revised: February 21, 2020
Accepted: March 10, 2020
Article in press: March 10, 2020
Published online: March 28, 2020
BACKGROUND
We studied the protective effects of Qingyi decoction (QYD) (a Traditional Chinese Medicine) against severe acute pancreatitis (SAP)-induced myocardial infarction (MI).
AIM
To study the function and mechanism of QYD in the treatment of myocardial injuries induced by SAP.
METHODS
Ultrasonic cardiography, hematoxylin and eosin staining, immunohistochemistry, qRT-PCR, western blot, enzyme-linked immunosorbent assays, and apoptosis staining techniques were used to determine the effects of QYD following SAP-induced MI in Sprague-Dawley rats.
RESULTS
Our SAP model showed severe myocardial histological abnormalities and marked differences in the symptoms, mortality rate, and ultrasonic cardiography outputs among the different groups compared to the control. The expression of serum cytokines [interleukin (IL)-1ß, IL-6, IL-8, IL-12, amyloid β, and tumor necrosis factor-α] were significantly higher in the SAP versus QYD treated group (P < 0.05 for all). STIM1 and Orai1 expression in myocardial tissue extracts were significantly decreased post QYD gavage (P < 0.001). There was no significant histological difference between the 2-aminoethyl diphenylborinate inhibitor and QYD groups. The SAP group had a significantly higher apoptosis index score compared to the QYD group (P < 0.001).
CONCLUSION
QYD conferred cardio-protection against SAP-induced MI by regulating myocardial-associated protein expression (STIM1 and Orai1).
Core tip: This manuscript illustrates the protective role of the Traditional Chinese Medicine, Qingyi decoction (QYD), on severe acute pancreatitis-induced myocardial injuries. QYD treatment was able to play a double role as a preventive and curative agent for both myocardial injuries and acute severe pancreatitis via the STIM1/Orai1-SOCE pathway. QYD treatment reduced myocardial injuries and the mortality rate by 30% in Sprague-Dawley (SD) rats within 24 h. Our findings reveal the success of QYD in improving the overall appearance of SD rats, together with decreasing the myocardial histological abnormalities and inflammatory cells infiltration caused by severe acute pancreatitis and myocardial injuries.
