Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model

Table 1 Group-wise description of mean laboratory measurements + standard deviations (bilirubin, γ-GT, ASAT, ALAT, albumin, quick), laboratory measurements + standard deviation of IL-33, p-21 and GADD45a mRNA, mean measurements + standard deviation of histology

	Control	7 d	14 d	21 d	28 d	42 d	56 d
Bilirubin (μmol/L)	2 ± 0	156.83 ± 34.12	37.34 ± 54.39	43.6 ± 61.49	20.5 ± 38.02	20 ± 40.25	2 ± 0
		P = 0.004	P = 0.004	P = 0.008	P = 0.429	P = 0.690	P = 1.000
Albumin (g/L)	8.4 ± 0.89	5.83 ± 0.41	7 ± 1.09	7.6 ± 1.34	8 ± 1.22	9.25 ± 0.96	9.2 ± 0.84
		P = 0.004	P = 0.052	P = 0.421	P = 0.329	P = 0.690	P = 0.222
Quick (%)	127 ± 3.13	137 ± 10.33	126 ± 5.02	126 ± 8.94	128 ± 4.49	96 ± 30.89	107 ± 4.79
		P = 0.052	P = 1.000	P = 0.548	P = 0.429	P = 0.095	P = 0.016
ASAT (μmol/L.s)	0.91 ± 0.06	5.9 ± 0.66	2.1 ± 2.19	4.05 ± 2.55	1.9 ± 1.94	2.06 ± 1.82	1.07 ± 0.23
		P = 0.004	P = 0.030	P = 0.008	P = 0.009	P = 0.151	P = 0.095
ALAT (μmol/L.s)	0.74 ± 0.09	2.11 ± 0.29	1.03 ± 0.38	1.95 ± 0.48	1.09 ± 0.18	0.93 ± 0.24	0.96 ± 0.15
		P = 0.004	P = 0.030	P = 0.008	P = 0.004	P = 0.310	P = 0.032
γ-GT (μmol/L.s)	0.07 ± 0	0.4 ± 0.09	0.22 ± 0.36	0.35 ± 0.31	0.12 ± 0.11	0.12 ± 0.12	0.07 ± 0
		P = 0.004	P = 0.662	P = 0.151	P = 0.662	P = 0.690	P = 1.000
GADD45a (GADD45a/GAPDH mRNA)	1 ± 0.58	0.84 ± 0.15	0.91 ± 0.35	3.25 ± 0.36	2.37 ± 1	0.99 ± 0.38	0.9 ± 0.43
		P = 0.841	P = 0.931	P = 0.008	P = 0.030	P = 1.000	P = 0.393
p-21 (p21/GAPDH mRNA)	1 ± 0.64	7.06 ± 1.8	7.1 ± 2.55	1.93 ± 1.19	3.04 ± 1.33	1.52 ± 1.44	3.07 ± 1.24
		P = 0.008	P = 0.004	P = 0.151	P = 0.009	P = 0.841	P = 0.036
IL-33 (IL33/GAPDH mRNA)	1 ± 0.21	0.91 ± 0.2	1.33 ± 0.44	4.14 ± 1.93	2.41 ± 0.82	2.58 ± 0.17	1.23 ± 0.26
		P = 0.841	P = 0.329	P = 0.008	P = 0.004	P = 0.008	P = 0.036
Bile ducts (bile ducts per PF)		8 ± 0.65	17 ± 3.47	48 ± 6.13	14 ± 2.76	4 ± 0.24	5 ± 0.46
			P = 0.002	P = 0.004	P = 0.015	P = 0.126	P = 0.004
Necrosis (% per slide)		0.29 ± 0.14	1.38 ± 2.28	1.31 ± 1.03	0.47 ± 0.21	0.09 ± 0.07	0.21 ± 0.2
			P = 0.002	P = 0.004	P = 0.065	P = 0.004	P = 0.662
Fibrosis (% per slide)		1.09 ± 0.61	1.3 ± 0.9	1.22 ± 1.33	2.32 ± 1.67	1.76 ± 0.61	1.53 ± 0.83
			P = 1.000	P = 1.000	P = 0.132	P = 0.247	P = 1.000
ASDCL (PF) (positive cells per PF)		36 ± 5.38	43 ± 13	34 ± 2.15	22 ± 5.93	11 ± 1.74	6 ± 1.01
			P = 0.394	P = 0.931	P = 0.041	P = 0.004	P = 0.004
HMGB-1 (PF) (positive cells per PF)		17 ± 7.07	18 ± 7.67	12 ± 5.91	13 ± 5.2	8 ± 4.39	6 ± 3.78
			P = 0.394	P = 0.329	P = 0.310	P = 0.009	P = 0.004
HMGB-1 (SF) (positive cells per FoV)		25 ± 13.1	13 ± 8.18	10 ± 4.17	9 ± 8.43	5.4 ± 3.42	4 ± 3.09
			P = 0.065	P = 0.052	P = 0.002	P = 0.009	P = 0.004

P values of Mann-Whitney-U-Test (P < 0.05).