Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Nov 7, 2005; 11(41): 6459-6465
Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6459
Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6459
Table 1 WBC and PMN cells count (/µL, mean±SD)
Group | WBC after 5 h | WBC after 9 h | PMN after 5 h | PMN after 9 h |
(/µL) | (/µL) | (/µL) | (/µL) | |
Control | 5 120 ± 1 220 | 5 597 ± 1 447 | 1230 ± 490 | 1 434 ± 569 |
AP | 2 204a± 705a | 4 225 ±583 | 614a ± 229a | 1 286 ± 598 |
AP+MoAb | 3 942c± 493c | 4 037 ±1 778 | 2 315c±850c | 1 120 ± 705 |
AP+LPS | 1 678 ± 259 | 6 762c±580c | 1 047 ± 560 | 5 146c±1 124c |
AP+MoAb | 4 087 ± 259 | 5 450 ± 580 | 1 830 ± 560 | 3 250 ± 1 124 |
+LPS | ||||
MoAb | 6 587±1 003 | 3 887 ± 1 612 | 2 200 ± 502 | 990 ± 307 |
LPS | 3 771a±1 510a | 4 100 ± 1 824 | 2 892a±1 372a | 2 959a±1 553a |
Table 2 SH compound and MDA concentration within pancreatic homogenate (nmol/mg protein, mean±SD)
Group | SH after 5 h | H after 9 h | MDA after 5 h | MDA after 9 h |
Control | 60.3 ± 7.3 | 64.7 ± 8.3 | 0.21 ± 0.02 | 0.22 ± 0.03 |
AP | 40.2a ± 8.5a | 43.5a ± 4.3a | 0.38a ± 0.07a | 0.39a ± 0.03a |
AP+MoAb | 40.0 ± 6.5 | 46.7 ± 1.4 | 0.25c ± 0.06c | 0.26c ± 0.03c |
AP+LPS | 40.0±6.8 | 41.1 ± 8.2 | 0.39 ± 0.07 | 0.41 ± 0.1 |
AP+MoAb | 39.0 ± 6.7 | 40.4 ± 5.0 | 0.37 ± 0.08 | 0.35 ± 0.2 |
+LPS | ||||
MoAb | 63.4 ± 8.6 | 59.4 ± 9.0 | 0.2 ± 0.03 | 0.21 ± 0.04 |
LPS | 52.0 ± 9.1 | 48.6 ± 4.7 | 0.28 ± 0.04 | 0.35 ± 0.05 |
Table 3 Plasma α-amylase and lipase activity (U/L, mean ± SD)
Group | Α-Amylase | Α-Amylase | Lipase | Lipase |
after 5 h | after 9 h | after 5 h | after 9 h | |
Control | 690 ± 120 | 743 ± 156 | 6 ± 8 | 8 ± 9 |
AP | 8 286a ± 143a | 5 908a ± 640a | 2 140a ± 1 123a | 929a ± 252a |
AP+MoAb | 5 119 ± 2 487 | 2 293c ± 1 739c | 1 704 ± 731 | 342c ± 178c |
AP+LPS | 9 310 ± 7 552 | 4 001 ± 1 638 | 2 573 ± 2 133 | 1 257 ± 164 |
AP+MoAb | 12 699c ± 4 978c | 2 719c ± 634c | 3 470 ± 1 832 | 305c ± 65c |
+LPS | ||||
MoAb | 696 ± 151 | 582 ± 273 | 21 ± 12 | 8 ± 4 |
LPS | 584 ± 182 | 332 ± 93 | 11 ± 8 | 8 ± 4 |
Table 4 Plasma IL-6 concentration (pg/mL, mean ± SD)
Table 5 Microscopic findings within the pancreatic gland (mean ± SD)
Houvs | Edema | Vacuolization | Infiltration | Necrosis | ||||
5 | 9 | 5 | 9 | 5 | 9 | 5 | 9 | |
Group 1 | 0.00 | 0.00 | 0.00 | 0.00 | 1.1 | 1.21 | 0.00 | 0.00 |
Control | ±0.00 | ±0.00 | ±0.00 | ±0.00 | ±0.9 | ±1.03 | ±0.00 | ±0.00 |
Group 2 | 1.91a | 0.50 | 2.50a | 1.00 | 7.40a | 14.60a | 0.17 | 0.25 |
AP | ±0.90a | ±0.57 | ±0.90a | ±1.41 | ±5.29a | ±2.37a | ±0.38 | ±0.50 |
Group 3 | 2.28 | 1.50 | 1.71 | 1.50 | 3.87c | 10.95 | 0.00 | 0.00 |
AP+MoAb CD11b | ±0.75 | ±1.00 | ±0.4 8 | ±1.00 | ±1.69c | ±0.92 | ±0.00 | ±0.00 |
Group 4 | 1.28 | 2.00 | 1.57 | 1.25 | 10.94 | 17.92 | 0.14 | 0.00 |
AP+LPS | ±1.25 | ±1.15 | ±1.39 | ±1.25 | ±3.02 | ±5.98 | ±0.37 | ±0.00 |
Group 5 | 2.50 | 1.00 | 2.00 | 1.00 | 10.40 | 15.10 | 0.00 | 0.00 |
AP+MoAbCD11b +LPS | ±1.00 | ±0.00 | ±1.15 | ±0.00 | ±8.30 | ±6.46 | ±0.00 | ±0.00 |
Group 6 | 0.25 | 0.00 | 0.00 | 0.00 | 0.80 | 0.25 | 0.00 | 0.00 |
Control+MoAb CD11b | ±0.50 | ±0.00 | ±0.00 | ±0.00 | ±1.04 | ±0.30 | ±0.00 | ±0.00 |
Group 7 | 0.00 | 0.00 | 0.00 | 0.00 | 2.45 | 6.00a | 0.00 | 0.00 |
Control+LPS | ±0.00 | ±0.00 | ±0.00 | ±0.00 | ±2.75 | ±4.11a | ±0.00 | ±0.00 |
- Citation: Hać S, Dobosz M, Kaczor JJ, Rzepko R, Aleksandrowicz-Wrona E, Wajda Z, Śledziński Z, Krajewski J. Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats. World J Gastroenterol 2005; 11(41): 6459-6465
- URL: https://www.wjgnet.com/1007-9327/full/v11/i41/6459.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i41.6459