Hać S, Dobosz M, Kaczor JJ, Rzepko R, Aleksandrowicz-Wrona E, Wajda Z, Śledziński Z, Krajewski J. Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats. World J Gastroenterol 2005; 11(41): 6459-6465 [PMID: 16425416 DOI: 10.3748/wjg.v11.i41.6459]
Corresponding Author of This Article
Stanisłasw Hać, MD, PhD, Department of General Endocrine and Transplant Surgery, Medical University of Gdańsk, 7 Dębinki Street, Gdańsk 80-952, Poland. sthac@amg.gda.pl
Article-Type of This Article
Basic Research
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World J Gastroenterol. Nov 7, 2005; 11(41): 6459-6465 Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6459
Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats
Stanisław Hać, Marek Dobosz, Jan J Kaczor, Robert Rzepko, Ewa Aleksandrowicz-Wrona, Zdzisław Wajda, Zbigniew Śledziński, Jacek Krajewski
Stanisław Hać, Zbigniew Śledziński, Jacek Krajewski, Department of General Endocrine and Transplant Surgery, Medical University of Gdańsk, Poland
Marek Dobosz, Department of General and Gastroenterological Surgery, St. Vincent a’ Paulo Hospital of Gdynia, Poland
Jan J Kaczor, Department of Bioenergetics, J. Śniadecki University School of Physical Education in Gdańsk, Poland
Robert Rzepko, Department of Pathomorphology, Medical University of Gdańsk, Poland
Ewa Aleksandrowicz-Wrona, Department of Clinical Nutrition, Medical University of Gdańsk, Poland
Zdzisław Wajda, II Department of General Gastroenterological and Endocrinological Surgery Medical University of Gdańsk, Poland
Author contributions: All authors contributed equally to the work.
Supported by the grant from Medical University of Gdańsk, Poland
Correspondence to: Stanisłasw Hać, MD, PhD, Department of General Endocrine and Transplant Surgery, Medical University of Gdańsk, 7 Dębinki Street, Gdańsk 80-952, Poland. sthac@amg.gda.pl
Telephone: +48-58-3492410 Fax: +48-58-3492410
Received: March 7, 2005 Revised: April 2, 2005 Accepted: April 4, 2005 Published online: November 7, 2005
Abstract
AIM: To investigate the influence of neutrophil adhesion molecule blockade with monoclonal antibody (MoAb CD11b) and E. coli lipopolysaccharide (LPS) administration on experimental acute pancreatitis (AP).
METHODS: AP was induced by four ip injections of cerulein (Cn) at 1-h intervals. MoAb CD 11b and LPS were administered at the beginning of the experiment.
RESULTS: The neutrophil count and chemiluminescence were diminished at the beginning of AP. The oxidative stress parameters were found within the pancreatic gland. MoAb CD 11b used for AP resulted in a significant reduction of pancreatic infiltration and pancreatitis oxidative stress parameters. Serum interleukin-6 (IL-6) was not detected in AP animals, whereas high serum IL-6 concentration was noted only in animals receiving LPS.
CONCLUSION: Neutrophils are involved in pancreatic damage in the early stage of AP. Neutrophil infiltration reduction protects the pancreatic gland from destruction during AP. LPS does not change the early course of Cn pancreatitis in rats.
