Alterations in expression, proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma

Table 1 Primers and PCR reactions for multi-region amplifications of clusterin

Amplified fragments (bp)	Sense primers	Antisense primers	PCR conditions
1-1350	5’-CCGGATCCTTATGATGAAGACTCTGCTGCTG-3’	5’-GCCTCGAGTCACTCCCGCTGCTTTTTG-3’	95°C for 40 s, 62°C for 40 s, and 72°Cfor 90 s
1-1056	5’-CCGGATCCTTATGATGAAGACTCTGCTGCTG-3’	5’-GTGTTGAGCATCTTCCACTG-3’	95°C for 30 s, 60°C for 40 s, and 72°C for 60 s
1-321	5’-CCGGATCCTTATGATGAAGACTCTGCTGCTG-3’	5’-CATCATGGTCTCATTGCACAC-3’	95°C for 30 s, 56°C for 30 s, and 72°C for 30 s
437-1056	5’-GAGCTCGCCCTTCTACTTCT-3’	5’-GTGTTGAGCATCTTCCACTG-3’	95°C for 30 s, 56°C for 30 s, and 72°C for 30 s
437-1350	5’-GAGCTCGCCCTTCTACTTCT-3’	5’-GCCTCGAGTCACTCCCGCTGCTTTTTG-3’	95°C for 40 s, 60°C for 40 s, and 72°C for 60 s
1120-1350	5’-ACCTCACGCAAGGCGAAGACC-3’	5’-TCTCACTCCTCCCGGTGCTT-3’	95°C for 30 s, 56°C for 25 s, and 72°C for 25 s
GAPDH	5’-ACCACAGTCCATGCCATCAC-3’	5’-TCCACCACCCTGTTGCTGTA-3’