He HZ, Song ZM, Wang K, Teng LH, Liu F, Mao YS, Lu N, Zhang SZ, Wu M, Zhao XH. Alterations in expression, proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma. World J Gastroenterol 2004; 10(10): 1387-1391 [PMID: 15133840 DOI: 10.3748/wjg.v10.i10.1387]
Corresponding Author of This Article
Xiao-Hang Zhao, M.D., Ph.D. National Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. zhaoxh@pubem.cicams.ac.cn
Article-Type of This Article
Esophageal Cancer
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World J Gastroenterol. May 15, 2004; 10(10): 1387-1391 Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1387
Alterations in expression, proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma
Hong-Zhi He, Zhen-Mei Song, Kun Wang, Liang-Hong Teng, Fang Liu, You-Sheng Mao, Ning Lu, Shang-Zhong Zhang, Min Wu, Xiao-Hang Zhao
Hong-Zhi He, Liang-Hong Teng, Fang Liu, You-Sheng Mao, Ning Lu, Min Wu, Xiao-Hang Zhao, National Laboratory of Molecular Oncology, Department of Thoracic Surgery and Department of Pathology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Zhen-Mei Song, Shang-Zhong Zhang, Qilu Hospital, School of Medicine, Shandong University, Qindao 250012, Shandong Province, China
Kun Wang, Xiao-Hang Zhao, Beijing Yanjing Hospital, Beijing 100037, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation, No.30225045, No.39990570, No.30171049 and No.30370713, and National High Tech and Major State Basic R & D Program of China, No.G1998051205 and No.2001AA227091
Correspondence to: Xiao-Hang Zhao, M.D., Ph.D. National Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. zhaoxh@pubem.cicams.ac.cn
Telephone: +86-10-67709015 Fax: +86-10-67709015
Received: January 9, 2004 Revised: February 17, 2004 Accepted: February 24, 2004 Published online: May 15, 2004
Abstract
AIM: To investigate biogenesis and intracellular localizations of clusterin to elucidate the potential molecular mechanisms implicated in tumorigenesis of esophageal mucosa.
METHODS: Semi-quantitative RT-PCR for multi-region alteration analysis, Western blot for different transcriptional forms and immunohistochemical staining for intracellular localizations of clusterin were carried out in both tissues and cell lines of ESCC.
RESULTS: The N-terminal deletions of the clusterin gene and the appearance of a 50-53 ku nuclear clusterin, an uncleaved, nonglycosylated, and disulfide-linked isoform, were the major alterations in cancer cells of esophagus. Naturally the 40 ku clusterin was located in the connective tissue of the lamina propria of epithelial mucosa and right under the basal membrane of epithelia, but it was disappeared in stromal mucosa of esophagus and the pre-matured clusterin was found positive in cancerous epithelia.
CONCLUSION: The N-terminal deletion of clusterin may be essential for its alterations of biogenesis in ESCC.
