Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 61-61
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.61
Regulation of telomerase activity by recombinant human tumor necrosis factor in vitro
Wei-Fen Xie, Yong Lin, Zhong-Bing Zhang, Xing-Rong Zhang, Wei-Zhong Chen, Jian-Wei Shen, Hao Wang, Department of Gastroenterology, Changzheng Hospital, Second Millitary Medical University, Shanghai 200003, China
Xin Zhang, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Wei-Fen Xie, Department of Gastroenterology, Changzheng Hospital, Second Millitary Medical University, 415 Fengyang Road, Shanghai 200003, China. Weifenxie@yahoo.com
Telephone: 21-63610109-73255
Received: May 6, 2000
Revised: June 14, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To investigate the effect of recombinant human tumor necrosis factor (rhTNF) on telomerase activity in hepatoma cell line HepG2 and HepG1-6.

METHODS: TRAP-ELISA method was used to determine the telomerase activity in HepG2 and HepG1-6 cells which were treated by different concent rations of rhTNF. In addition, the TERTLuc (800) plasmid was transiently transfected, which was inserted 800 bp of the human telomerase reverse transcriptase (hTERT) promoter, into HepG2 cells by Lipofect. Different concentrations of rhTNF were added into the culture media 2 h later, and the activity of the hTERT promoter was detected 48 h after transfection.

RESULTS: The telomerase activity of HepG2 was suppressed by rhTNF in a dose-dependent manner. The results also revealed that the activity of hTERT promoter was inhibited linearly with rhTNF at the dose of 10-1000 IU/mL.

CONCLUSION: Inhibition of the hTERT promoter expression by rhTNF may contribute to its anti-tumor activity.

Key Words: Tumor necrosis factor; Telomerase activity; Liposomes; Transfection; Liver neoplasms; In vitro



Footnotes

E- Editor: Zhang FF

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