Regulation of telomerase activity by recombinant human tumor necrosis factor in vitro

doi:10.3748/wjg.v6.iSuppl3.61

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Sep 15, 2000 (publication date) through Nov 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 61-61
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.61

Regulation of telomerase activity by recombinant human tumor necrosis factor in vitro

Wei-Fen Xie, Yong Lin, Zhong-Bing Zhang, Xing-Rong Zhang, Wei-Zhong Chen, Xin Zhang, Jian-Wei Shen, Hao Wang

Wei-Fen Xie, Yong Lin, Zhong-Bing Zhang, Xing-Rong Zhang, Wei-Zhong Chen, Jian-Wei Shen, Hao Wang, Department of Gastroenterology, Changzheng Hospital, Second Millitary Medical University, Shanghai 200003, China

Xin Zhang, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Wei-Fen Xie, Department of Gastroenterology, Changzheng Hospital, Second Millitary Medical University, 415 Fengyang Road, Shanghai 200003, China. Weifenxie@yahoo.com

Telephone: 21-63610109-73255

Received: May 6, 2000
Revised: June 14, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To investigate the effect of recombinant human tumor necrosis factor (rhTNF) on telomerase activity in hepatoma cell line HepG2 and HepG1-6.

METHODS: TRAP-ELISA method was used to determine the telomerase activity in HepG2 and HepG1-6 cells which were treated by different concent rations of rhTNF. In addition, the TERTLuc (800) plasmid was transiently transfected, which was inserted 800 bp of the human telomerase reverse transcriptase (hTERT) promoter, into HepG2 cells by Lipofect. Different concentrations of rhTNF were added into the culture media 2 h later, and the activity of the hTERT promoter was detected 48 h after transfection.

RESULTS: The telomerase activity of HepG2 was suppressed by rhTNF in a dose-dependent manner. The results also revealed that the activity of hTERT promoter was inhibited linearly with rhTNF at the dose of 10-1000 IU/mL.

CONCLUSION: Inhibition of the hTERT promoter expression by rhTNF may contribute to its anti-tumor activity.

Keywords: Tumor necrosis factor; Telomerase activity; Liposomes; Transfection; Liver neoplasms; In vitro