Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 142-142
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.142
Construction and expression of a fusion protein consisting anti-HBsAg antibody fragment Fab and interferon-a in E. coli
Wei Zheng, Hua Tan, Shao-Bai Song, Yan-Xing Yu, Ru Yin, Department of General Surgery, General Hospital of PLA, Beijing 100853, China
Hao-Ying Lu, The Academy of Military Medicine, Beijing 100781, China
Yan Wang, General Hospital of Navy, Beijing 100410, China
Supported by The National Natural Science Foundation of China, No. 39670678
Correspondence to: Dr. Wei Zheng, Department of General Surgery, General Hospital of PLA, Beijing 100853, China. zhengwei301@fm365.com
Telephone: 10-66939762
Received: October 3, 1999
Revised: November 26, 1999
Accepted: January 28, 2000
Published online: September 15, 2000

Abstract

AIM: To construct an expression vector for anti-HBsAg antibody Fab fragment and interferon-aA (IFN-aA) fusion protein in E. coli.

METHODS: With PCR and molecular clone techniques, we amplified the gene fragment of IFN-aA with corresponding endonuclease sites and artificial linker at 5’, 3’ termini, and then formed pHS/IFN-aA by recombining it within the vector in correct endonuclease sites, choosing the positive clone to transform into E. coli and introduced by IPTG to express the fusion protein.

RESULTS: Enzymic hydrolysis and DNA sequence measurement confirmed that human gene of IFN-aA was correctly cloned to the vector and could express fusion protein in E. coli.

CONCLUSION: The success in construction and expression of a fusion protein makes it possible to carry out further studies on its purification and targeted polypeptide therapy to HB virus.

Key Words: Interferon-alpha; Immunoglobulin fragments; Viral fusion proteins; Hepatitis B virus; Polymerase chain reaction; Escherichia coli



Footnotes

E- Editor: Zhang FF

$[References]