Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.142
Revised: November 26, 1999
Accepted: January 28, 2000
Published online: September 15, 2000
AIM: To construct an expression vector for anti-HBsAg antibody Fab fragment and interferon-aA (IFN-aA) fusion protein in E. coli.
METHODS: With PCR and molecular clone techniques, we amplified the gene fragment of IFN-aA with corresponding endonuclease sites and artificial linker at 5’, 3’ termini, and then formed pHS/IFN-aA by recombining it within the vector in correct endonuclease sites, choosing the positive clone to transform into E. coli and introduced by IPTG to express the fusion protein.
RESULTS: Enzymic hydrolysis and DNA sequence measurement confirmed that human gene of IFN-aA was correctly cloned to the vector and could express fusion protein in E. coli.
CONCLUSION: The success in construction and expression of a fusion protein makes it possible to carry out further studies on its purification and targeted polypeptide therapy to HB virus.