Expression properties of recombinant pEgr-P16 plasmid in esophageal squamous cell carcinoma induced by ionizing irradiation

Figure 1 Diagram of the construction of the plasmid pEgr-P16.

Figure 2 Expression of P16 in EC9706 cells after γ-irradiation. Lane 1: control; Lane 2: 0Gy; Lane 3: 2Gy; Lane 4: 4Gy; Lane 5: 8Gy; Lane 6: 10Gy; Lane 7: 20Gy.

Figure 3 Amplification curves and post-amplification disso-ciation curves for P16 in EC9706 cells.

Figure 4 Expression of P16 in EC9706 cells at different time points after 2Gy γ-irradiation, ^aP < 0. 01 vs. 2 h group.

Figure 5 Apoptotic changes of transfected EC9706 cells. ^aP < 0.01 vs pcDNA3.1⁺group, ^bP < 0.001 vs control group, ^cP < 0.05 vs pcDNA3.1⁺ group.