Esophageal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2003; 9(12): 2650-2653
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2650
Expression properties of recombinant pEgr-P16 plasmid in esophageal squamous cell carcinoma induced by ionizing irradiation
Cong-Mei Wu, Tian-Hua Huang, Qing-Dong Xie, De-Sheng Wu, Xiao-Hu Xu
Cong-Mei Wu, Tian-Hua Huang, Qing-Dong Xie, Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, China
Xiao-Hu Xu, Department of Forensic Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, China
De-Sheng Wu, Lanzhou Medical College, Lanzhou 730000, Gansu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30210103904 and the Science and Technology Program of Guangdong Province, No.2003C30304
Correspondence to: Dr. Tian-Hua Huang, Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, China. thhuang@stu.edu.cn
Telephone: +86-754-8900442 Fax: +86-754-8557562
Received: June 21, 2003
Revised: June 26, 2003
Accepted: July 24, 2003
Published online: December 15, 2003
Abstract

AIM: To construct the recombinant pEgr-P16 plasmid for the investigation of its expression properties in esophageal squamous cell carcinoma induced by ionizing irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma.

METHODS: The recombinant pEgr-P16 plasmid was constructed and transfected into EC9706 cells with lipofectamine. Western blot, quantitative RT-PCR and flow cytometry were performed to study the expression of pEgr-P16 in EC9706 cells and the biological characteristics of EC9706 cell line after transfection induced by ionizing irradiation.

RESULTS: The eukaryotic expression vector pEgr-P16 was successfully constructed and transfected into EC9706 cells. The expression of P16 was significantly increased in the transfected cells after irradiation while the transfected cells were not induced by ionizing irradiation. The induction of apoptosis in transfection plus irradiation group was higher than that in plasmid alone or irradiation alone.

CONCLUSION: The combination of pEgr-P16 and irradiation could significantly enhance the P16 expression property and markedly induce apoptosis in EC9706 cells. These results may lay an important experimental basis for gene radiotherapy for esophageal carcinoma.

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