Esophageal Cancer
Copyright ©The Author(s) 2003.
World J Gastroenterol. Nov 15, 2003; 9(11): 2404-2408
Published online Nov 15, 2003. doi: 10.3748/wjg.v9.i11.2404
Figure 1
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Figure 1 pAd-p27kip1 appraisal 1200 bp ladder Marker: 200, 400, 600, 800, 1000, 1200, 1400, 1600, 1800, 2000 bp. 2, pCMV5p27kip1 (kpn I + BamH I): 690 bp segment. 3, pAd-p27kip1 (kpn I + BamH I): 690 bp and 8800 bp segment. 4, pACCMVPLPA (kpn I + BamH I): 8800 bp segment. 5, Hind III marker: 2027, 2322, 4361, 6557, 9416, 23130 bp.
Figure 2
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Figure 2 Ad-p27kip1 PCR appraisal 1200 bp ladder Marker: 200, 400, 600, 800, 1000, 1200, 1400, 1600, 1800, 2000 bp, 2, pCMV5p27kip1model, 275 bp gene segament, 3, Ad-p27kip1 model: 275 bp and 860 bp gene segament, 4, PAVVMVPLPA model, 860 bp gene segament.
Figure 3
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Figure 3 Growth curve of cell of esophageal carcinoma.
Figure 4
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Figure 4 p27kip1 overexpression in transfection group × 200.
Figure 5
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Figure 5 p27 negative expression in control group × 200.
Figure 6
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Figure 6 DNA fragment analysis 1200 bp ladder marker: 200, 400, 600, 800, 1000, 1200, 1400, 1600, 1800, 2000 bp. 2, Ad-p27kip1 group: 2 μg. 3, Ad-p27kip1 group: 1 μg. 4. Blank group.