Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain

Figure 1 Amplification of variable genes of immunoglobulin light and heavy chains with PCR. The PCR products were resolved on 1.2% agarose gel.

Figure 2 The nucleotide and amino acid sequences of 3H11 mAb VL(a) and VH(b) genes. Underlined sequences are CDR regions. Numbering according to mature protein sequences.

Figure 3 Human-mouse chimeric light chain expression vector pAG4622.