Li J, Wang Y, Li QX, Wang YM, Xu JJ, Dong ZW. Cloning of 3H11 mAb variable region gene and expression of 3H11 human-mouse chimeric light chain. World J Gastroenterol 1998; 4(1): 41-44 [PMID: 11819228 DOI: 10.3748/wjg.v4.i1.41]
Corresponding Author of This Article
Dr. Jing Li, Department of Oncology Molecular Immunology, Beijing Institute for Cancer Research, Beijing Medical University, Dahongluochang Street, Xicheng District, Beijing 100034, China
Article-Type of This Article
Original Articles
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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Jing Li, Yan Wang, Quan-Xi Li, Ya-Ming Wang, Jian-Jun Xu, College of Oncology, Beijing Medical University, Beijing 100034, China
Yan Wang, The Navy General Hospital, Beijing 100037, China
Zhi-Wei Dong, Institute for Cancer Research, Chinese Academy Medical Sciences, Beijing 100021, China
Jing Li, male, born on 1971-09-29 in Xinxiang City, Henan Province, attending Beijing Medical University for PhD.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Jing Li, Department of Oncology Molecular Immunology, Beijing Institute for Cancer Research, Beijing Medical University, Dahongluochang Street, Xicheng District, Beijing 100034, China
Telephone: +86-10-68587733-58238
Received: April 14, 1997 Revised: September 20, 1997 Accepted: October 19, 1997 Published online: February 15, 1998
Abstract
AIM: To clone mouse anti-human gastric cancer mAb (3H11) variable genes and to construct 3H11 human-mouse chimericantibody.
METHODS: The entire VH and VL genes of anti-gastric cancer mAb 3H11 were cloned by RT-PCR method from 3H11 hybridoma cells, using 5’ primers for leader sequences. The 3H11 VL gene was then inserted into human-mouse chimeric light chain expression vector and transfected into murine Sp2/0 myeloma cells.
RESULTS: DNA sequence analysis indicated that the cloned genes included the whole leader sequences and the mature Ig variable region encoding sequences. Aftergene transfection, transient expression of chimeric light chain protein was detected.
CONCLUSION: DNA sequences and transient expression indicated that the cloned gene was functional. This work laid basis for constructing 3H11 human-mouse chimeric antibody in the future.
