Qingyi decoction protects against myocardial injuries induced by severe acute pancreatitis

Figure 1 Qingyi decoction impairs cardiac dysfunction in a rat model of severe acute pancreatitis. Echocardiography was used to assess cardiac function. A: Representative M-mode images; B: Left ventricular ejection fraction; C: Left ventricular fractional shortening. Results in panel B and C are expressed as the mean ± SE (n = 10 for each group). ^aP ≤ 0.05, sham group vs the indicated group; ^cP ≤ 0.05, severe acute pancreatitis group vs the indicated group. SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate; LVEF: Left ventricular ejection fraction; LVFS: Left ventricular fractional shortening.

Figure 2 Hematoxylin and eosin staining of left ventricular myocardial tissues suggests that Qingyi decoction attenuates myocardial injury. Representative images of hematoxylin and eosin staining of tissue sections from the Sham, severe acute pancreatitis, 2-aminoethyl diphenylborinate, and Qingyi decoction groups (magnification, 40×). SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate.

Figure 3 Apoptosis analysis of myocardial cells in the Sham, severe acute pancreatitis, 2-aminoethyl diphenylborinate, and Qingyi decoction groups, as determined by TUNEL assay (magnification, 40×). Representative TUNEL assay results are shown; apoptotic cells are stained brown/yellow (orange arrows) and non-apoptotic cells are stained blue. SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate.

Figure 4 Effect of Qingyi decoction on STMI1 and Orai1 expression in myocardial tissues following severe acute pancreatitis. A: Quantitative RT-PCR analysis of STMI1; B: Quantitative RT-PCR analysis of Orai1; C: Western blot representative image; D: Quantification relative to GAPDH. Values are expressed as the mean ± SE (n = 10 for each group). ^ashows significant difference (P ≤ 0.05) between sham group and the indicated group; ^bshows significant difference (P ≤ 0.05) between severe acute pancreatitis group and the indicated group; ^cshows significant difference (P ≤ 0.05) between 2-aminoethyl diphenylborinate group and the indicated group. SAP: Severe acute pancreatitis; QYD: Qingyi decoction; 2-APB: 2-Aminoethyl diphenylborinate; STIM1: Stromal interaction molecule 1; ORAI1: Calcium release-activated calcium channel protein 1.