Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases

Figure 1 Panel of immunohistochemical markers. Ki67 in A: Cirrhosis (low); B: Grade 2 (low); C: Grade 3 (moderate); D: Grade 4 (high). K19 in E: Cirrhosis (negative in hepatocytes); F: Grade 3 (focal); G: Grade 3 (moderate); H: Grade 4 (moderate); p53 in I: Cirrhosis (negative); J: Grade 2 (focal); K: Grade 3 (moderate); L: Grade 3 (diffuse). ERK1 in M: Cirrhosis (negative); N: Grade 2 (weak); O: Grade 3 (moderate); P: Grade 3 (strong). Beta-catenin in Q: Cirrhosis (strong membrane expression); R: Grade 2 (weak membrane); S: Grade 3 (strong membrane); T: Grade 4 (nuclear expression). ERK2 in U: Cirrhosis (negative in hepatocytes and positive in endothelial cells); V: Grade 2 (negative); W: Grade 3 (moderate); X: Grade 3 (strong). Original magnification × 400.

Figure 2 Panel of immunohistochemical markers. Vimentin in A: Cirrhosis (negative in hepatocytes and positive in sinusoids); B: Grade 2 (negative in hepatocytes and positive in sinusoids); C: Grade 3 (focally positive); D: Grade 4 (focally positive in small cell component). Cyclin-D1 in E: Cirrhosis (focal); F: Grade 2 (focal); G: Grade 3 (moderate); H: Grade 4 (moderate); mTOR in I: Cirrhosis (focal); J: Grade 2 (negative); K: Metastasis grade 3 (weak); L: Grade 4 (strong). MET in M: Cirrhosis (moderate); N: Grade 2 (weak); O: Grade 3 (moderate); P: Grade 3 (negative). EGFR in Q: Cirrhosis (moderate - score 140); R: Grade 2 (score 200); S: Grade 3 (score 60); T: Grade 4 (score 0). Caspase 3 in U: Cirrhosis (moderate); V: Grade 2 (moderate); W: Grade 3 (weak); X: Grade 3 (negative/loss). Original magnification × 400.