Case Control Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 21, 2016; 22(27): 6246-6256
Published online Jul 21, 2016. doi: 10.3748/wjg.v22.i27.6246
Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases
Aloísio Felipe-Silva, Alda Wakamatsu, Cinthya dos Santos Cirqueira, Venâncio Avancini Ferreira Alves
Aloísio Felipe-Silva, LIM 14-Patologia Hepática, Faculdade de Medicina da USP, 01246-903 São Paulo SP, Brazil
Aloísio Felipe-Silva, Alda Wakamatsu, Cinthya dos Santos Cirqueira, Venâncio Avancini Ferreira Alves, Department of Pathology, Medical Research Laboratory LIM14, University of Sao Paulo School Of Medicine, 01246-903 São Paulo SP, Brazil
Author contributions: Felipe-Silva A designed and performed the research, analyzed the data, and wrote the manuscript; Wakamatsu A and dos Santos Cirqueira C contributed optimal technical support on construction of the tissue microarray and performance of the histological and immunohistochemistry reactions; and Alves VAF analyzed the data, wrote the manuscript, and supervised all the research steps.
Supported by Fundação de Amparo a Pesquisa do Estado de São Paulo, No. 08/58855-3 to Alves VAF.
Institutional review board statement: This research was approved by the Ethics Committee for Research Project Analysis (CAPPesq) of the Hospital das Clínicas of the University of Sao Paulo School Of Medicine (Protocol No. 2346-0484/08 on 06/24/08).
Conflict-of-interest statement: None of the authors has any potential or real conflict of interest related to this study or the publication of its findings. This study reports major findings of a PhD thesis by the first author performed at the University of Sao Paulo and was partially presented at the 2014 United States and Canadian Academy of Pathology (USCAP) Annual Meeting in San Diego, USA.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Aloísio Felipe-Silva, MD, PhD, LIM 14-Patologia Hepática, Faculdade de Medicina da USP, Avenida Dr. Arnaldo 455 1o. Andar Sala 1157, 01246-903 São Paulo SP, Brazil. aloisiosilva@hu.usp.br
Telephone: +55-11-30617433 Fax: +55-11-30617234
Received: March 22, 2016
Peer-review started: March 23, 2016
First decision: April 14, 2016
Revised: May 19, 2016
Accepted: June 15, 2016
Article in press: June 15, 2016
Published online: July 21, 2016
Processing time: 114 Days and 23.9 Hours
Core Tip

Core tip: This study assessed the immunohistochemistry-detected expression of several protein products of genes known to be involved in the molecular pathogenesis of hepatocellular carcinoma (HCC) in a retrospective autopsy cohort of patients with HCC. The data showed that expression profiles of these markers may be related to different pathways underlying HCC progression and metastasis, and that the Edmondson-Steiner’s tumor grade may reflect currently recognized molecular subclasses of HCC. This cross-sectional analysis supports the strategy of translating genomic data into panels of immunohistochemical markers for risk evaluation in HCC and also reinforces the paramount importance of histological grade in this context.