Inhibition of transfected PTEN on human colon cancer

Figure 1 Digested plasmid PTEN-pMD18-T 1: DL15000 marker; 2: pcDNA-PTEN-WT doubly digested; 3: pcDNA-PTEN-MT doubly digested; 4: pcDNA-PTEN-WT PCR; 5: product of pcDNA-PTEN-MT PCR.

Figure 2 Growth curve of four cells. PTEN-1 (PTEN-wild type), PTEN-2 (PTEN-mutant type), PcDNA3.1 (vector).

Figure 3 Detection of Western wild type and mutant type PTEN genes 1: transfected cell pcDNA-PTEN-WT; 2: transfected cell pcDNA-PTEN-MT; 3: transfected vacant vector cell pcDNA3. 1 (+); 4: nontransfected cells.

Figure 4 Result of LoVo cell cycle after transfection by flow cytometry.

Figure 5 Result of apoptotic LoVo cells after transfection in-duced by 5-Fu.