Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2004; 10(24): 3670-3673
Published online Dec 15, 2004. doi: 10.3748/wjg.v10.i24.3670
Inhibition of transfected PTEN on human colon cancer
Shou-Shui Xu, Wen-Lu Shen, Song-Ying Ouyang
Shou-Shui Xu, Wen-Lu Shen, Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, Guangdong Province, China
Song-Ying Ouyang, Faculty of Military Medicine, Quartermaster University of PLA, Changchun 130062, Jilin Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Postdoctor Shou-Shui Xu, Shantou University Medical College, Shantou 515031, Guangdong Province, China. smile4612003@yahoo.com.cn
Telephone: +86-754-8900466
Received: February 11, 2004
Revised: February 26, 2004
Accepted: March 4, 2004
Published online: December 15, 2004
Abstract

AIM: To study the inhibitory effect of transfected PTEN on LoVo cells.

METHODS: Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT.

RESULTS: PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells.

CONCLUSION: Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.

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