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©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2022; 28(25): 2920-2936
Published online Jul 7, 2022. doi: 10.3748/wjg.v28.i25.2920
Published online Jul 7, 2022. doi: 10.3748/wjg.v28.i25.2920
Fecal gene detection based on next generation sequencing for colorectal cancer diagnosis
Si-Yu He, Hai-Lin Peng, Cheng-Lin Zhou, Mei Lin, Department of Clinical Laboratory, Taizhou People's Hospital (Postgraduate Training Base of Dalian Medical University), Taizhou 225300, Jiangsu Province, China
Si-Yu He, Department of Clinical Laboratory, The First People's Hospital of Tianmen City, Tianmen 431700, Hubei Province, China
Ying-Chun Li, Yong Wang, Department of General Surgery, Taizhou People's Hospital (Postgraduate Training Base of Dalian Medical University), Taizhou 225300, Jiangsu Province, China
Chuan-Meng Zhang, Central Laboratory, Taizhou People's Hospital (Postgraduate training base of Dalian Medical University), Taizhou 225300, Jiangsu Province, China
Sheng-Lan Chen, Department of Laboratory, Taizhou Genewill Medical Laboratory Company Limited, Taizhou 225300, Jiangsu Province, China
Jian-Feng Yin, Department of Laboratory, Jiangsu CoWin Biotech Co., Ltd., Taizhou 225300, Jiangsu Province, China
Author contributions: He SY performed most of the experiments, analyzed some data, and drafted the manuscript; Li YC and Wang Y provided specimens for the study; Peng HL and Zhou CL gave constructive guidance on the study; Zhang CM contributed to the statistics; Chen SL and Yin JF performed some of the experiments; Lin M designed and supervised the study and edited the manuscript; all authors approved the final version of the article.
Supported by Taizhou Social Development Plan , No. TS202004 ; Natural Science Foundation of Nanjing University of Chinese Medicine China , No. XZR2020093 ; and Taizhou People's Hospital Medical Innovation Team Foundation , No. CXTDA201901 .
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Taizhou people's Hospital in Jiangsu Province (No. KY201912501).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Mei Lin, MD, PhD, Senior Scientist, Department of Clinical Laboratory, Taizhou People's Hospital (Postgraduate Training Base of Dalian Medical University), No. 366 Taihu Road, Taizhou 225300, Jiangsu Province, China. l_mei@163.com
Received: January 16, 2022
Peer-review started: January 16, 2022
First decision: March 8, 2022
Revised: March 18, 2022
Accepted: May 27, 2022
Article in press: May 27, 2022
Published online: July 7, 2022
Peer-review started: January 16, 2022
First decision: March 8, 2022
Revised: March 18, 2022
Accepted: May 27, 2022
Article in press: May 27, 2022
Published online: July 7, 2022
Core Tip
Core Tip: Colorectal cancer (CRC) is characterized by high morbidity and mortality, as well as low early diagnosis rate. The development of current gold standard for clinical diagnosis of CRC is restricted due to its invasiveness. The purpose of this study is to explore the potential value of fecal gene detection based on next generation sequencing in the diagnosis of CRC, to screen specific genes or gene combinations suitable for CRC diagnosis and prognosis prediction in fecal DNA, and to establish a technical platform for fecal DNA detection for CRC screening, diagnosis, and efficacy monitoring.