Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 21, 2021; 27(43): 7530-7545
Published online Nov 21, 2021. doi: 10.3748/wjg.v27.i43.7530
Genome-wide map of N6-methyladenosine circular RNAs identified in mice model of severe acute pancreatitis
Jun Wu, Xiao-Hui Yuan, Wen Jiang, Yi-Chen Lu, Qi-Lin Huang, Yi Yang, Hua-Ji Qie, Jiang-Tao Liu, Hong-Yu Sun, Li-Jun Tang
Jun Wu, Xiao-Hui Yuan, Wen Jiang, Yi-Chen Lu, Qi-Lin Huang, Yi Yang, Hua-Ji Qie, Jiang-Tao Liu, Hong-Yu Sun, Li-Jun Tang, Department of General Surgery and Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command, Chengdu 610036, Sichuan Province, China
Jun Wu, Xiao-Hui Yuan, Wen Jiang, Yi-Chen Lu, Hua-Ji Qie, Jiang-Tao Liu, Hong-Yu Sun, Li-Jun Tang, College of Medicine, Southwest Jiaotong University, Chengdu 610036, Sichuan Province, China
Hong-Yu Sun, Laboratory of Basic Medicine, The General Hospital of Western Theater Command, Chengdu 610036, Sichuan Province, China
Author contributions: Wu J and Yuan XH contributed equally to this work; Tang LJ and Sun HY participated in the study conception and design; Wu J and Yuan XH participated in the writing of the main manuscript; Wu J, Yuan XH, Jiang W and Lu YC participated in the performance of the experiments; Huang QL and Yang Y participated in statistical data analysis and interpretation; Qie HJ and Liu JT participated in the preparation of figures; Tang LJ and Sun HY participated in the revision of the manuscript and final approval.
Supported by the National Natural Science Foundation of China, No. 81772001; and the National Clinical Key Subject of China, No. 41732113.
Institutional review board statement: The study was reviewed and approved by the Institutional Ethics Committee at the General Hospital of Western Theater Command (Chengdu, China), No. A20190252005.
Institutional animal care and use committee statement: The experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee at the General Hospital of Western Theater Command (Chengdu, China), and were conducted in accordance with the established International Guiding Principles for Animal Research.
Conflict-of-interest statement: The authors declare that there is no conflict of interest related to this study.
Data sharing statement: We had submitted the data to the online repository, which can be found at: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE173298.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Li-Jun Tang, MD, PhD, Chief Doctor, Professor, Department of General Surgery and Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command, No. 270 Tianhuan Road, Furong Avenue, Jinniu District, Chengdu 610036, Sichuan Province, China. tanglj2016@163.com
Received: May 19, 2021
Peer-review started: May 19, 2021
First decision: June 22, 2021
Revised: June 23, 2021
Accepted: September 15, 2021
Article in press: September 15, 2021
Published online: November 21, 2021
Processing time: 183 Days and 19.7 Hours
Core Tip

Core Tip: We identified a transcriptome-wide map of N6-methyladenosine (m6A) circRNAs and determined their biological significance and potential mechanisms in severe acute pancreatitis (SAP). The main findings were: (1) Function analysis found that circRNAs with differentially expressed m6A peaks were involved in the key process of SAP; (2) m6A may affect the interplays of circRNAs and microRNAs to participate in the pathogenesis of SAP; and (3) Demethylase alkylation repair homolog 5 may play key roles in dynamic process of m6A to downregulate the total m6A level of circRNAs in SAP. We provided novel insights to explore the possible pathophysiological mechanism of SAP and seek new potential therapeutic targets.