Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 28, 2021; 27(16): 1785-1804
Published online Apr 28, 2021. doi: 10.3748/wjg.v27.i16.1785
Depletion of MRPL35 inhibits gastric carcinoma cell proliferation by regulating downstream signaling proteins
Ling Yuan, Jia-Xin Li, Yi Yang, Yan Chen, Ting-Ting Ma, Shuang Liang, Yang Bu, Lei Yu, Yi Nan
Ling Yuan, Jia-Xin Li, Yi Yang, Ting-Ting Ma, Pharmacy College of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Ling Yuan, Yi Nan, Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Yan Chen, Traditional Chinese Medicine College, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Shuang Liang, Department of Oncology and Endocrinology, Yinchuan Hospital of Traditional Chinese Medicine Affiliated to Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Yang Bu, Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Lei Yu, Department of Infectious Diseases, The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Author contributions: Nan Y provided the conceptual and technical guidance, designed the study, and revised the manuscript critically for important intellectual content; Yuan L carried out most of in vivo studies, analyzed the data, and wrote the manuscript; Li JX carried out all of in vitro experiments and wrote the manuscript; Yang Y and Ma TT performed parts of in vivo studies; Chen Y conducted statistical analysis of all of the data; Liang S, Bu Y, and Yu L supervised the clinical relevance and coordinated the clinic pathological features; all authors have read and approved the manuscript.
Supported by Ningxia Natural Science Foundation, No. 2020AAC03130.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Ningxia Medical University (No. 2020-071).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Ningxia Medical University (IACUC protocol number: 2019-083).
Conflict-of-interest statement: All authors declare no financial or commercial conflict of interest.
Data sharing statement: All data generated or analyzed during this study are included in this published article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yi Nan, MD, PhD, Professor, Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, Ningxia Medical University, No. 1160 Shengli Street, Yinchuan 750004, Ningxia Hui Autonomous Region, China. 20080011@nxmu.edu.cn
Received: December 22, 2020
Peer-review started: December 22, 2020
First decision: January 23, 2021
Revised: February 4, 2021
Accepted: March 11, 2021
Article in press: March 11, 2021
Published online: April 28, 2021
Processing time: 119 Days and 18 Hours
Core Tip

Core Tip: MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein. Our results showed that compared with adjacent tissues, the expression of MRPL35 in gastric carcinoma (GC) tissues was increased significantly, which was related to age, lymph node metastasis, and pathological tumor-node-metastasis stage of GC patients. Knockdown of MRPL35 inhibited GC cell proliferation, clone formation, and tumor formation in BALB/c nude mice, induced apoptosis, reduced the expression of PICK1 and BCL-XL proteins, and increased that of AGR2 protein. These data indicate that MRPL35 might be an oncogene and be used as a new therapeutic target for GC.