Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 21, 2017; 23(23): 4211-4221
Published online Jun 21, 2017. doi: 10.3748/wjg.v23.i23.4211
Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal
Mohamed Amine Zaouali, Arnau Panisello-Roselló, Alexandre Lopez, Carlos Castro Benítez, Emma Folch-Puy, Agustín García-Gil, Teresa Carbonell, René Adam, Joan Roselló-Catafau
Mohamed Amine Zaouali, Arnau Panisello-Roselló, Emma Folch-Puy, Joan Roselló-Catafau, Experimental Hepatic Ischemia-Reperfusion Unit, Institut d’Investigacions Biomèdiques de Barcelona, Spanish National Research Council, 08036 Barcelona, Spain
Mohamed Amine Zaouali, Research Unit of Biology and molecular anthropology applied to development and Health (UR12ES11), Faculty of Pharmacy, University of Monastir, Monastir 5000, Tunisia
Mohamed Amine Zaouali, Tunisia and Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, Tunisia
Alexandre Lopez, Carlos Castro Benítez, René Adam, Centre Hépatobiliaire, AP-PH, Hôpital Paul Brousse, 94804 Paris, France
Agustín García-Gil, Hospital Clínico de Zaragoza, 50009 Zaragoza, Spain
Teresa Carbonell, Facultat Biologia, Universitat de Barcelona, 08028 Barcelona, Catalonia, Spain
Author contributions: Zaouali MA and Panisello-Roselló A contributed equally to this study and carried out the experimental work; Lopez A, Folch-Puy E and Castro Benítez C provided protocols and analyzed data; Panisello-Roselló A, García-Gil A, Carbonell T and Adam R established the animal experimental model used in this study and contributed to the critical analyses of the data; Zaouali MA, Panisello-Roselló A and Roselló-Catafau J designed the study, coordinated the experiments and wrote the paper.
Supported by Instituto de Salud Carlos III (ISCIII) through the FIS project PI12/0056, co-funded by FEDER from Regional Development European Funds (European Union).
Conflict-of-interest statement: None of the authors have any conflict of interest to declare related to this paper.
Data sharing statement: Technical data are available from the corresponding author at jrcbam@iibb.csic.es. Participants gave informed consent for data sharing.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Joan Roselló-Catafau, Experimental Hepatic Ischemia-Reperfusion Unit, Institut d’Investigacions Biomèdiques de Barcelona, Spanish National Research Council, C/Rosselló 161, 7th floor, 08036 Barcelona, Spain. joan.rosello@iibb.csic.es
Telephone: +34-93-3638333 Fax: +34-93-3638301
Received: January 25, 2017
Peer-review started: February 1, 2017
First decision: March 16, 2017
Revised: April 8, 2017
Accepted: June 1, 2017
Article in press: June 1, 2017
Published online: June 21, 2017
Processing time: 146 Days and 11.5 Hours
Core Tip

Core tip: Although several reports have confirmed that proteolytic activity during cold storage determines graft outcome after transplantation, the effect of preservation solution on steatotic liver graft proteolysis and on the activation of ATP-dependent proteasome during cold ischemia injury has not been fully investigated. Here, we compared the effect of two preservation solutions Institut Georges Lopez-1(IGL-1) and University of Wisconsin on liver proteolysis and ubiquitin-proteasome activation when steatotic liver grafts were subjected to cold storage. We provide evidence for a protective role of proteasome and proteolysis inhibition using IGL-1 during steatotic liver graft preservation.