Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 7, 2016; 22(17): 4345-4353
Published online May 7, 2016. doi: 10.3748/wjg.v22.i17.4345
Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells
Lei Li, Hong-Hai Hong, Shi-Ping Chen, Cai-Qi Ma, Han-Yan Liu, Ya-Chao Yao
Lei Li, Hong-Hai Hong, Shi-Ping Chen, Han-Yan Liu, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
Cai-Qi Ma, Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Ya-Chao Yao, Department of Clinical Laboratory Medicine, the Second People’s Hospital of Guangdong Province, Guangzhou 510317, Guangdong Province, China
Author contributions: Li L, Hong HH and Chen SP contributed equally to this study; Li L, Hong HH and Chen SP carried out the experiments and drafted the manuscript; Ma CQ participated in the experiments; Liu HY analysed the data; Yao YC designed the study and directed its implementation; all authors made critical revisions related to the intellectual content of the manuscript, and approved the final version of the article to be published.
Supported by National Nature Science Foundation of China, No. 81400639 and No. 81502507; The Doctoral Start-up Foundation of Guangzhou Medical University of China, No. 2014C39; The Science Foundation for Youth Scientists of the Second People’s Hospital of Guangdong Province of China, No. YQ2015-002.
Conflict-of-interest statement: To the best of the authors knowledge, all the listed authors have participated actively in the study, and have seen and approved the submitted manuscript. The authors do not have any possible conflicts of interest.
Data sharing statement: No additional unpublished data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Ya-Chao Yao, PhD, Department of Clinical Laboratory Medicine, the Second People’s Hospital of Guangdong Province, No. 466 Xinggangzhonglu, Haizhu District, Guangzhou 510317, Guangdong Province, China.
Telephone: +86 020 89168136
Received: December 6, 2015
Peer-review started: December 7, 2015
First decision: January 28, 2016
Revised: February 25, 2016
Accepted: March 14, 2016
Article in press: March 14, 2016
Published online: May 7, 2016
Core Tip

Core tip: Hepatitis B virus markedly protected the cells from growth suppression and cell death in the condition of serum deprivation. A decrease of superoxide anion production accompanied with an increase of manganese superoxide dismutase (MnSOD) expression and activity was found in HepG2.215 cells. Moreover, AMP-activated protein kinase activation contributed to the up-regulation of MnSOD. Hepatitis B virus X protein was identified to promote the expression of AMPK and MnSOD.