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World J Gastroenterol. Apr 28, 2014; 20(16): 4778-4786
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4778
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4778
Proteomic analysis of liver mitochondria from rats with nonalcoholic steatohepatitis
Lin Li, You-Ming Li, Xue-Qun Zhang, Xin-Xin Zhou, Xi Jin, Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
De-Zhao Lu, College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310003, Zhejiang Province, China
Author contributions: Li L and Lu DZ performed the majority of experiments; Li YM, Zhang XQ and Zhou XX provided vital regents and analytical tools and were also involved in editing the manuscript; Jin X designed the study and wrote the manuscript.
Supported by National Natural Science Foundation of China No. 81000169, No. 81100277, No. 81370008, and No. 81200284; the Excellent Young Investigator Foundation of the Health Bureau of Zhejiang Province No. 2010QNA011; the Excellent Young Investigator Natural Science Foundation of Zhejiang Province No. R2110159; the Project of Zhejiang Traditional Chinese Medicine Administration Bureau No. 2010ZA065; and the Fundamental Research Funds for the Central Universities No. 2013QNA702
Correspondence to: Dr. Xi Jin, the First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jxfl007@hotmail.com
Telephone: +86-571-87236603 Fax: +86-571-87236611
Received: January 3, 2014
Revised: February 7, 2014
Accepted: March 5, 2014
Published online: April 28, 2014
Processing time: 115 Days and 17.2 Hours
Revised: February 7, 2014
Accepted: March 5, 2014
Published online: April 28, 2014
Processing time: 115 Days and 17.2 Hours
Core Tip
Core tip: Nonalcoholic fatty liver disease (NAFLD) is a major worldwide cause of chronic liver diseases, and nonalcoholic steatohepatitis (NASH) plays a critical role as a “turning point” in the development of NAFLD. Nevertheless, the pathogenesis of NASH remains unclear, and mitochondrial dysfunction is known to be actively involved. To date, no study has reported on specific protein expression patterns in NASH mitochondria. We have, for the first time, performed a proteomic analysis of mitochondria from NASH rats, aiming to provide a protein reservoir for in-depth analyses of NASH mechanisms and for the exploration of potential therapeutics.