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World J Gastroenterol. Apr 28, 2014; 20(16): 4692-4701
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4692
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4692
Localization and vasopressin regulation of the Na+-K+-2Cl- cotransporter in the distal colonic epithelium
Hong Xue, Xiao-Shuang Li, Hai-Mei Sun, Qian Kang, Bo Wu, Ya-Xi Wang, Wan-Jing Zou, De-Shan Zhou, Department of Histology and Embryology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China
Zi-Juan Zhang, Experiment Teaching Center, School of Basic Medical Science, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China
Author contributions: Xue H, Wang YX, Kang Q and Li XS performed the experiments; Xue H, Zhang ZJ and Sun HM contributed to the reagents; Xue H, Wu B and Zou WJ collected the data; Zhou DS and Xue H designed the research and wrote the paper.
Supported by National Natural Science Foundation of China, No. 31271290 and No. 31000514; Scientific Research Key Program of Beijing Municipal Commission of Education No. KZ201310025020; Beijing Postdoctoral Research Foundation; Beijing Natural Science Foundation No. 7142025
Correspondence to: De-Shan Zhou, MD, Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Xitoutiao 10, Beijing 100069, China. zhouds08@ccmu.edu.cn
Telephone: +86-10-83950079 Fax: +86-10-83911449
Received: January 30, 2014
Revised: February 9, 2014
Accepted: March 5, 2014
Published online: April 28, 2014
Revised: February 9, 2014
Accepted: March 5, 2014
Published online: April 28, 2014
Core Tip
Core tip: The Na+-K+-2Cl- cotransporter (NKCC2), which was thought to be only expressed in the apical membrane of the epithelial cells in the thick ascending limb of Henle’s loop, was recently found to be expressed in the colon. However, the role and regulating mechanism of NKCC2 in the gut are still not completely understood. Our results provide direct evidence that vasopressin also plays an important role in the colonic epithelia by stimulating NKCC2 trafficking to the apical membrane and inducing NKCC2-mediated ion transport. The action of vasopressin on NKCC2 in the colon would be recognized to supplement the role of the kidney in modulating whole-body homeostasis and electrolyte balance in physiological or pathophysiologic conditions.