Nong FF, Liang YQ, Xing SP, Xiao YF, Chen HH, Wen B. Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1. World J Gastroenterol 2022; 28(35): 5154-5174 [PMID: 36188720 DOI: 10.3748/wjg.v28.i35.5154]
Corresponding Author of This Article
Bin Wen, PhD, Academic Research, Full Professor, Pi-Wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510000, Guangdong Province, China. wenbin@gzucm.edu.cn
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Sep 21, 2022; 28(35): 5154-5174 Published online Sep 21, 2022. doi: 10.3748/wjg.v28.i35.5154
Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1
Fei-Fei Nong, Yin-Fang Xiao, Hui-Hui Chen, Bin Wen, Pi-Wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China
Yu-Qi Liang, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China
Shang-Ping Xing, Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, School of Pharmacy, Guangxi Medical University, Nanning 530022, Guangxi Zhuang Autonomous Region, China
Author contributions: Wen B designed the experiments; Nong FF and Liang YQ performed the experiments and wrote the paper; Xing SP revised the paper; Xiao YF and Chen HH analyzed the data; all authors have read and approved this final manuscript.
Supported bythe National Natural Science Foundation of China, No. 81673944.
Institutional review board statement: All human subjects were approved by the Ethics Committee of The First Affiliated Hospital of Guangzhou University of Chinese Medicine [No. Y (2019) 172].
Institutional animal care and use committee statement: All animal protocols were approved by the Animal Care and Use Committee of the Guangzhou University of Chinese Medicine (Permit No. 20190801066).
Conflict-of-interest statement: The authors state that the study was conducted in the absence of any business or financial relationships that could be interpreted as potential conflicts of interest.
Data sharing statement: The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Bin Wen, PhD, Academic Research, Full Professor, Pi-Wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510000, Guangdong Province, China. wenbin@gzucm.edu.cn
Received: May 25, 2022 Peer-review started: May 25, 2022 First decision: July 12, 2022 Revised: July 23, 2022 Accepted: August 22, 2022 Article in press: August 22, 2022 Published online: September 21, 2022 Processing time: 112 Days and 23.3 Hours
ARTICLE HIGHLIGHTS
Research background
Colorectal cancer (CRC) is a common malignant tumor.
Research motivation
Alcohol consumption is positively correlated with CRC malignant metastasis; however, the mechanism is unclear.
Research objectives
To investigate the effects of alcohol on CRC metastasis
Research methods
The interaction between laminin-γ2 (LAMC2) and integrin-β1 (ITGB1) was measured by Duolink assay, and the expression levels of LAMC2, ITGB1 and focal adhesion kinase, snail, fibronectin, N-cadherin and special AT-rich sequence binding protein 1 were measured by quantitative real-time polymerase chain reaction, immunohistochemistry and western blotting. Interleukin-1β (IL-1β), tumor necrosis factor-α and IL-6 levels were measured via enzyme-linked immunosorbent assay, histopathological assessment via hematoxylin eosin staining, and determination of aberrant crypt foci via methylene blue.
Research results
Alcohol may promote CRC metastasis by influencing the molecular mechanism of the premetastatic niche.
Research conclusions
Our study suggests that alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of CRC by activating the early interaction between LAMC2 and ITGB1 and lead to CRC metastasis.
Research perspectives
To explore the effect of alcohol on colorectal cancer metastasis.
