Li YF, Sun HX, Wu GD, Du WN, Zuo J, Shen Y, Qiang BQ, Yao ZJ, Wang H, Huang W, Chen Z, Xiong MM, Meng Y, Fang FD. Protein kinase C/ζ (PRKCZ) Gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes. World J Gastroenterol 2003; 9(9): 2078-2082 [PMID: 12970910 DOI: 10.3748/wjg.v9.i9.2078]
Corresponding Author of This Article
Fu-De Fang and Yan Meng, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. fangfd@public3.bta.net.cn, ymengsmile@yahoo.com
Article-Type of This Article
Basic Research
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Sep 15, 2003; 9(9): 2078-2082 Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2078
Protein kinase C/ζ (PRKCZ) Gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes
Yun-Feng Li, Hong-Xia Sun, Guo-Dong Wu, Wei-Nan Du, Jin Zuo, Yan Shen, Bo-Qin Qiang, Zhi-Jian Yao, Heng Wang, Wei Huang, Zhu Chen, Mo-Miao Xiong, Yan Meng, Fu-De Fang
Yun-Feng Li, Hong-Xia Sun, Guo-Dong Wu, Wei-Nan Du, Jin Zuo, Yan Meng, Fu-De Fang, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
Yan Shen, Bo-Qin Qiang, Zhi-Jian Yao, Chinese National Human Genome Center at Beijing, Beijing, 100176, China
Wei Huang, Zhu Chen, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China
Heng Wang, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China
Mo-Miao Xiong, Human Genetics Center, Health Science Center, The University of Texas, Houston TX 77225, USA
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39896200, No30170441, the National High Technology Research and Development Program, No. 2001AA221161, No. 2002BA711A05, No. 2002BA711A10-02. The National Program for Key Basic Research Projects, No. G1998051016, the Natural Science Foundation of Beijing, No. 7002026
Correspondence to: Fu-De Fang and Yan Meng, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. fangfd@public3.bta.net.cn, ymengsmile@yahoo.com
Telephone: +86-10-65253005 Fax: +86-10-65253005
Received: January 18, 2003 Revised: January 29, 2003 Accepted: March 3, 2003 Published online: September 15, 2003
Abstract
AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.
METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed.
RESULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P < 0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.
CONCLUSION: PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.
