Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2003; 9(9): 2040-2044
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2040
Gadolinium chloride and salvia miltiorrhiza compound ameliorate reperfusion injury in hepatocellular mitochondria
Wen-Hai Zhang, Jin-Sheng Wang, Yong Zhou, Jian-Yi Li
Wen-Hai Zhang, Jin-Sheng Wang, Yong Zhou, Jian-Yi Li, Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110022, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Science Foundation of Liaoning Province, No. 619025
Correspondence to: Dr. Wen-Hai Zhang, Department of General Surgery, Second Affiliated Hospital, China Medical University, 39. Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China. surgeonzwh@163.net
Telephone: +86-24-25943022
Received: March 28, 2003
Revised: April 23, 2003
Accepted: May 11, 2003
Published online: September 15, 2003
Abstract

AIM: To investigate the effect of gadolinium chloride (GaCl3) and salvia miltiorrhiza compound (SMCo) on ischemia and reperfusion (I/R) injury in hepatocellular mitochondria.

METHODS: Wistar rats were randomly to divided into control group, GaCl3 group, SMCo group and GaCl3 + SMCo group (n = 15 each). GaCl3 (7 mg·kg-1) was injected into tail vein on d 1 and d 2 in contrast group. SMCo (2 mL·kg-1) was injected into muscle on d 1 and d 2 in SMCo group. GaCl3 + SMCo group received both GaCl3 (iv) and SMCo (im) injection. Control group received saline injection only. On d 3, all the rats were subjected to 2 h ischemia in the middle and left lobes of the liver, followed by reperfusion for 2 h, 6 h and 18 h respectively. The level of serum alanine aminotransferase (ALT) and malondialdehyde (MDA) in hepatocellular mitochondria was measured. Pathological changes in hepatic tissue and in hepatocellular mitochondria were determined with optical microscope and electronic microscope, respectively.

RESULTS: Remarkablly pathohistological and biochemical changes were detected after 6 h of I/R. Compared with control, the level of ALT was decreased in GaCl3, SMCo and GaCl3 + SMCo treated groups (1314.0 ± 278.7 vs 809.4 ± 196.1, 716.6 ± 242.8 and 837.2 ± 190.6 IU·L-1, respectively. P < 0.05). Similarly, the level of MDA was decreased in GaCl3, SMCo and GaCl3 + SMCo treated groups (293.1 ± 51.1 vs 190.8 ± 55.5, 214.3 ± 32.9 and 221.0 ± 47.3 nmol·g-1, respectively, P < 0.05). Accordingly, in control group, swelling, degeneration, focal necrosis, infiltration of leucocyte were found in reperfused tissue under an optical microscope, and mitochondria swelling, rupture and even breakdown were seen under an electronic microscope. These pathohistological and ultrastructural damages caused by I/R were greatly attenuated in GaCl3, SMCo and GaCl3 + SMCo treated groups. However, there was no additive effect observed when GaCl3 and SMCo were used together.

CONCLUSION: Both GaCl3 and SMCo can alleviate the I/R injury in hepatocellular mitochondria.

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